BBa_I729009
type: ComponentDefinition
persistent identity: BBa I729009
display id: BBa_I729009
version: 1
was generated by: iGEM to SBOL conversion
description: Time device
created: 2008-09-17T11:00:00Z
modified: 2015-08-31T04:07:55Z
mutable provenance: Biobricks
owned by: james
owned by: myers
mutable description: Time device only AHL -> death add aTc until the lux-lac hybrid promoter activate -> express GFP
discontinued: true
dominant: false
experience: experience/None
group u list: _189_
m user id: 0
owner id: 1585
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: None
star: false
is replaced by: BBa_K084011
creator: Hiroki Fukutomi
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0010
component: BBa_E0040
component: BBa_I751502
component: BBa_B0012
component: BBa_K145151
component: BBa_B0033
component: BBa_J23105
component: BBa_C0040
component: BBa_B0034
component: BBa_R0040
component: BBa_C0012
sequence annotation: BBa_J23105
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_C0012
sequence annotation: BBa_E0040
sequence annotation: BBa_I751502
sequence annotation: BBa_B0033
sequence annotation: BBa_K145151
sequence: https://synbiohub.org/public/igem/BBa_I729009_sequence/1

BBa_B0010
type: Component
persistent identity: component1976330
display id: component1976330
version: 1
top level: BBa_I729009
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1976329
display id: component1976329
version: 1
top level: BBa_I729009
definition: GFP
access: public

BBa_I751502
type: Component
persistent identity: component1976342
display id: component1976342
version: 1
top level: BBa_I729009
definition: BBa_I751502
access: public

BBa_B0012
type: Component
persistent identity: component1976332
display id: component1976332
version: 1
top level: BBa_I729009
definition: BBa_B0012
access: public

BBa_K145151
type: Component
persistent identity: component1976349
display id: component1976349
version: 1
top level: BBa_I729009
definition: ccdB
access: public

BBa_B0033
type: Component
persistent identity: component1976344
display id: component1976344
version: 1
top level: BBa_I729009
definition: BBa_B0033
access: public

BBa_B0012
type: Component
persistent identity: component1976352
display id: component1976352
version: 1
top level: BBa_I729009
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1976350
display id: component1976350
version: 1
top level: BBa_I729009
definition: BBa_B0010
access: public

BBa_J23105
type: Component
persistent identity: component1976302
display id: component1976302
version: 1
top level: BBa_I729009
definition: BBa_J23105
access: public

BBa_C0040
type: Component
persistent identity: component1976307
display id: component1976307
version: 1
top level: BBa_I729009
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component1976304
display id: component1976304
version: 1
top level: BBa_I729009
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1976311
display id: component1976311
version: 1
top level: BBa_I729009
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1976309
display id: component1976309
version: 1
top level: BBa_I729009
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1976321
display id: component1976321
version: 1
top level: BBa_I729009
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1976316
display id: component1976316
version: 1
top level: BBa_I729009
definition: p(tetR)
access: public

BBa_B0034
type: Component
persistent identity: component1976327
display id: component1976327
version: 1
top level: BBa_I729009
definition: BBa_B0034
access: public

BBa_C0012
type: Component
persistent identity: component1976323
display id: component1976323
version: 1
top level: BBa_I729009
definition: lacI
access: public

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation1976302
display id: annotation1976302
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976302/range1976302/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_I729009/annotation1976302/range1976302/1
type: Range
persistent identity: range1976302
display id: range1976302
version: 1
top level: BBa_I729009
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1976304
display id: annotation1976304
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976304/range1976304/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I729009/annotation1976304/range1976304/1
type: Range
persistent identity: range1976304
display id: range1976304
version: 1
top level: BBa_I729009
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1976307
display id: annotation1976307
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976307/range1976307/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I729009/annotation1976307/range1976307/1
type: Range
persistent identity: range1976307
display id: range1976307
version: 1
top level: BBa_I729009
direction: direction/reverse
start: 62
end: 721
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1976309
display id: annotation1976309
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976309/range1976309/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I729009/annotation1976309/range1976309/1
type: Range
persistent identity: range1976309
display id: range1976309
version: 1
top level: BBa_I729009
direction: direction/forward
start: 755
end: 834
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1976311
display id: annotation1976311
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976311/range1976311/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I729009/annotation1976311/range1976311/1
type: Range
persistent identity: range1976311
display id: range1976311
version: 1
top level: BBa_I729009
direction: direction/forward
start: 843
end: 883
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1976316
display id: annotation1976316
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976316/range1976316/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I729009/annotation1976316/range1976316/1
type: Range
persistent identity: range1976316
display id: range1976316
version: 1
top level: BBa_I729009
direction: direction/forward
start: 892
end: 945
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1976321
display id: annotation1976321
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976321/range1976321/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I729009/annotation1976321/range1976321/1
type: Range
persistent identity: range1976321
display id: range1976321
version: 1
top level: BBa_I729009
direction: direction/reverse
start: 954
end: 965
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1976323
display id: annotation1976323
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976323/range1976323/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I729009/annotation1976323/range1976323/1
type: Range
persistent identity: range1976323
display id: range1976323
version: 1
top level: BBa_I729009
direction: direction/forward
start: 972
end: 2099
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1976327
display id: annotation1976327
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976327/range1976327/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I729009/annotation1976327/range1976327/1
type: Range
persistent identity: range1976327
display id: range1976327
version: 1
top level: BBa_I729009
direction: direction/reverse
start: 2133
end: 2144
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1976329
display id: annotation1976329
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976329/range1976329/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I729009/annotation1976329/range1976329/1
type: Range
persistent identity: range1976329
display id: range1976329
version: 1
top level: BBa_I729009
direction: direction/reverse
start: 2151
end: 2870
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1976330
display id: annotation1976330
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976330/range1976330/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I729009/annotation1976330/range1976330/1
type: Range
persistent identity: range1976330
display id: range1976330
version: 1
top level: BBa_I729009
direction: direction/forward
start: 2879
end: 2958
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1976332
display id: annotation1976332
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976332/range1976332/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I729009/annotation1976332/range1976332/1
type: Range
persistent identity: range1976332
display id: range1976332
version: 1
top level: BBa_I729009
direction: direction/forward
start: 2967
end: 3007
orientation: inline

BBa_I751502
type: SequenceAnnotation
persistent identity: annotation1976342
display id: annotation1976342
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976342/range1976342/1
component: BBa_I751502

https://synbiohub.org/public/igem/BBa_I729009/annotation1976342/range1976342/1
type: Range
persistent identity: range1976342
display id: range1976342
version: 1
top level: BBa_I729009
direction: direction/reverse
start: 3016
end: 3089
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation1976344
display id: annotation1976344
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976344/range1976344/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_I729009/annotation1976344/range1976344/1
type: Range
persistent identity: range1976344
display id: range1976344
version: 1
top level: BBa_I729009
direction: direction/forward
start: 3098
end: 3108
orientation: inline

BBa_K145151
type: SequenceAnnotation
persistent identity: annotation1976349
display id: annotation1976349
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976349/range1976349/1
component: BBa_K145151

https://synbiohub.org/public/igem/BBa_I729009/annotation1976349/range1976349/1
type: Range
persistent identity: range1976349
display id: range1976349
version: 1
top level: BBa_I729009
direction: direction/reverse
start: 3115
end: 3420
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1976350
display id: annotation1976350
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976350/range1976350/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I729009/annotation1976350/range1976350/1
type: Range
persistent identity: range1976350
display id: range1976350
version: 1
top level: BBa_I729009
direction: direction/forward
start: 3429
end: 3508
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1976352
display id: annotation1976352
version: 1
top level: BBa_I729009
location: https://synbiohub.org/public/igem/BBa_I729009/annotation1976352/range1976352/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I729009/annotation1976352/range1976352/1
type: Range
persistent identity: range1976352
display id: range1976352
version: 1
top level: BBa_I729009
direction: direction/forward
start: 3517
end: 3557
orientation: inline

BBa_K084011
type: ComponentDefinition
persistent identity: BBa K084011
display id: BBa_K084011
version: 1
was generated by: iGEM to SBOL conversion
description: Time device
created: 2008-09-17T11:00:00Z
modified: 2015-07-09T01:40:04Z
mutable provenance: Biobricks
owned by: james
owned by: myers
mutable description: Time device only AHL -> death AHL + aTc (until the lux-lac hybrid promoter activate) -> express GFP
discontinued: true
dominant: true
experience: experience/None
group u list: _189_
m user id: 4206
owner id: 1585
owning group id: 9
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: None
star: false
creator: Hiroki Fukutomi
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0015
component: BBa_K145151
component: BBa_B0033
component: BBa_I751502
component: BBa_I13504
component: BBa_I730003
component: BBa_I739001
sequence annotation: BBa_I739001
sequence annotation: BBa_I730003
sequence annotation: BBa_I13504
sequence annotation: BBa_I751502
sequence annotation: BBa_B0033
sequence annotation: BBa_K145151
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K084011_sequence/1

BBa_B0015
type: Component
persistent identity: component2315137
display id: component2315137
version: 1
top level: BBa_K084011
definition: BBa_B0015
access: public

BBa_K145151
type: Component
persistent identity: component2315130
display id: component2315130
version: 1
top level: BBa_K084011
definition: ccdB
access: public

BBa_B0033
type: Component
persistent identity: component2315125
display id: component2315125
version: 1
top level: BBa_K084011
definition: BBa_B0033
access: public

BBa_I751502
type: Component
persistent identity: component2315123
display id: component2315123
version: 1
top level: BBa_K084011
definition: BBa_I751502
access: public

BBa_I13504
type: Component
persistent identity: component2315116
display id: component2315116
version: 1
top level: BBa_K084011
definition: BBa_I13504
access: public

BBa_I730003
type: Component
persistent identity: component2315106
display id: component2315106
version: 1
top level: BBa_K084011
definition: BBa_I730003
access: public

BBa_I739001
type: Component
persistent identity: component2315094
display id: component2315094
version: 1
top level: BBa_K084011
definition: BBa_I739001
access: public

BBa_I739001
type: SequenceAnnotation
persistent identity: annotation2315094
display id: annotation2315094
version: 1
top level: BBa_K084011
location: https://synbiohub.org/public/igem/BBa_K084011/annotation2315094/range2315094/1
component: BBa_I739001

https://synbiohub.org/public/igem/BBa_K084011/annotation2315094/range2315094/1
type: Range
persistent identity: range2315094
display id: range2315094
version: 1
top level: BBa_K084011
direction: direction/reverse
start: 1
end: 883
orientation: inline

BBa_I730003
type: SequenceAnnotation
persistent identity: annotation2315106
display id: annotation2315106
version: 1
top level: BBa_K084011
location: https://synbiohub.org/public/igem/BBa_K084011/annotation2315106/range2315106/1
component: BBa_I730003

https://synbiohub.org/public/igem/BBa_K084011/annotation2315106/range2315106/1
type: Range
persistent identity: range2315106
display id: range2315106
version: 1
top level: BBa_K084011
direction: direction/reverse
start: 892
end: 2124
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2315116
display id: annotation2315116
version: 1
top level: BBa_K084011
location: https://synbiohub.org/public/igem/BBa_K084011/annotation2315116/range2315116/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K084011/annotation2315116/range2315116/1
type: Range
persistent identity: range2315116
display id: range2315116
version: 1
top level: BBa_K084011
direction: direction/reverse
start: 2133
end: 3007
orientation: inline

BBa_I751502
type: SequenceAnnotation
persistent identity: annotation2315123
display id: annotation2315123
version: 1
top level: BBa_K084011
location: https://synbiohub.org/public/igem/BBa_K084011/annotation2315123/range2315123/1
component: BBa_I751502

https://synbiohub.org/public/igem/BBa_K084011/annotation2315123/range2315123/1
type: Range
persistent identity: range2315123
display id: range2315123
version: 1
top level: BBa_K084011
direction: direction/reverse
start: 3016
end: 3089
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2315125
display id: annotation2315125
version: 1
top level: BBa_K084011
location: https://synbiohub.org/public/igem/BBa_K084011/annotation2315125/range2315125/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_K084011/annotation2315125/range2315125/1
type: Range
persistent identity: range2315125
display id: range2315125
version: 1
top level: BBa_K084011
direction: direction/forward
start: 3098
end: 3108
orientation: inline

BBa_K145151
type: SequenceAnnotation
persistent identity: annotation2315130
display id: annotation2315130
version: 1
top level: BBa_K084011
location: https://synbiohub.org/public/igem/BBa_K084011/annotation2315130/range2315130/1
component: BBa_K145151

https://synbiohub.org/public/igem/BBa_K084011/annotation2315130/range2315130/1
type: Range
persistent identity: range2315130
display id: range2315130
version: 1
top level: BBa_K084011
direction: direction/reverse
start: 3115
end: 3420
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2315137
display id: annotation2315137
version: 1
top level: BBa_K084011
location: https://synbiohub.org/public/igem/BBa_K084011/annotation2315137/range2315137/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K084011/annotation2315137/range2315137/1
type: Range
persistent identity: range2315137
display id: range2315137
version: 1
top level: BBa_K084011
direction: direction/reverse
start: 3429
end: 3557
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_I751502
type: ComponentDefinition
persistent identity: BBa I751502
display id: BBa_I751502
version: 1
was generated by: iGEM to SBOL conversion
description: plux-lac hybrid promoter
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
star: false
creator: Kenichiro Iwasaki
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: luxR binding site
sequence annotation: plac
sequence annotation: -35
sequence annotation: O1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I751502_sequence/1

luxR binding site
type: SequenceAnnotation
persistent identity: annotation1955564
display id: annotation1955564
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I751502/annotation1955564/range1955564/1
type: Range
persistent identity: range1955564
display id: range1955564
version: 1
top level: BBa_I751502
direction: direction/forward
start: 1
end: 19
orientation: inline

plac
type: SequenceAnnotation
persistent identity: annotation1955559
display id: annotation1955559
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I751502/annotation1955559/range1955559/1
type: Range
persistent identity: range1955559
display id: range1955559
version: 1
top level: BBa_I751502
direction: direction/forward
start: 20
end: 74
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955560
display id: annotation1955560
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I751502/annotation1955560/range1955560/1
type: Range
persistent identity: range1955560
display id: range1955560
version: 1
top level: BBa_I751502
direction: direction/forward
start: 20
end: 26
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955562
display id: annotation1955562
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I751502/annotation1955562/range1955562/1
type: Range
persistent identity: range1955562
display id: range1955562
version: 1
top level: BBa_I751502
direction: direction/forward
start: 27
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955561
display id: annotation1955561
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I751502/annotation1955561/range1955561/1
type: Range
persistent identity: range1955561
display id: range1955561
version: 1
top level: BBa_I751502
direction: direction/forward
start: 42
end: 48
orientation: inline

O1
type: SequenceAnnotation
persistent identity: annotation1955563
display id: annotation1955563
version: 1
top level: BBa_I751502
location: https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I751502/annotation1955563/range1955563/1
type: Range
persistent identity: range1955563
display id: range1955563
version: 1
top level: BBa_I751502
direction: direction/forward
start: 49
end: 74
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
top level: BBa_B0033
direction: direction/forward
start: 7
end: 10
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa K145151
display id: BBa_K145151
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB coding region
created: 2008-08-06T11:00:00Z
modified: 2015-07-08T03:14:50Z
mutable provenance: P1010
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the ccdB (control of cell death) gene.
discontinued: true
dominant: false
experience: experience/None
group u list: _257_
m user id: 4206
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable notes: just one stop codon in the end
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145151_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1970251
display id: annotation1970251
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
type: Range
persistent identity: range1970251
display id: range1970251
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1970253
display id: annotation1970253
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
type: Range
persistent identity: range1970253
display id: range1970253
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 303
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1970252
display id: annotation1970252
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
type: Range
persistent identity: range1970252
display id: range1970252
version: 1
top level: ccdB
direction: direction/forward
start: 304
end: 306
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I13504
type: ComponentDefinition
persistent identity: BBa I13504
display id: BBa_I13504
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13504_sequence/1

BBa_B0034
type: Component
persistent identity: component1505067
display id: component1505067
version: 1
top level: BBa_I13504
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1505084
display id: component1505084
version: 1
top level: BBa_I13504
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1505074
display id: component1505074
version: 1
top level: BBa_I13504
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1505069
display id: component1505069
version: 1
top level: BBa_I13504
definition: GFP
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505067
display id: annotation1505067
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
type: Range
persistent identity: range1505067
display id: range1505067
version: 1
top level: BBa_I13504
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505069
display id: annotation1505069
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
type: Range
persistent identity: range1505069
display id: range1505069
version: 1
top level: BBa_I13504
direction: direction/forward
start: 19
end: 738
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505074
display id: annotation1505074
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
type: Range
persistent identity: range1505074
display id: range1505074
version: 1
top level: BBa_I13504
direction: direction/forward
start: 747
end: 826
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505084
display id: annotation1505084
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
type: Range
persistent identity: range1505084
display id: range1505084
version: 1
top level: BBa_I13504
direction: direction/forward
start: 835
end: 875
orientation: inline

BBa_I730003
type: ComponentDefinition
persistent identity: BBa I730003
display id: BBa_I730003
version: 1
was generated by: iGEM to SBOL conversion
description: ptetR-LacI repressor
created: 2007-08-19T11:00:00Z
modified: 2015-08-31T04:07:55Z
mutable provenance: Valencia iGEM 2007
owned by: james
owned by: myers
mutable description: ptetR-strong RBS-LacI repressor(LVA+) R0040.B0034.C0012 intermediate part for the construction of our 'comparator'. note that it bears no terminator sequence, as we aim to add a fluorescence protein as multicistronic parts.
discontinued: false
dominant: true
experience: experience/Works
group u list: _157_
m user id: 0
owner id: 1095
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Arnau Montagud Aquino
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_I730003_sequence/1

BBa_C0012
type: Component
persistent identity: component2245629
display id: component2245629
version: 1
top level: BBa_I730003
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component2245627
display id: component2245627
version: 1
top level: BBa_I730003
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2245621
display id: component2245621
version: 1
top level: BBa_I730003
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2245621
display id: annotation2245621
version: 1
top level: BBa_I730003
location: https://synbiohub.org/public/igem/BBa_I730003/annotation2245621/range2245621/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I730003/annotation2245621/range2245621/1
type: Range
persistent identity: range2245621
display id: range2245621
version: 1
top level: BBa_I730003
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2245627
display id: annotation2245627
version: 1
top level: BBa_I730003
location: https://synbiohub.org/public/igem/BBa_I730003/annotation2245627/range2245627/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I730003/annotation2245627/range2245627/1
type: Range
persistent identity: range2245627
display id: range2245627
version: 1
top level: BBa_I730003
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2245629
display id: annotation2245629
version: 1
top level: BBa_I730003
location: https://synbiohub.org/public/igem/BBa_I730003/annotation2245629/range2245629/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I730003/annotation2245629/range2245629/1
type: Range
persistent identity: range2245629
display id: range2245629
version: 1
top level: BBa_I730003
direction: direction/forward
start: 81
end: 1208
orientation: inline

BBa_I739001
type: ComponentDefinition
persistent identity: BBa I739001
display id: BBa_I739001
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)
created: 2007-09-26T11:00:00Z
modified: 2015-08-31T04:08:01Z
mutable provenance: synthetic DNA
owned by: james
owned by: myers
mutable description: The Biobrick encodes tetR (BBa_C0040) under control of the constitutive promoter (BBa_J23100) followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2208
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: expression cassette for tetR
star: true
creator: Christian Kemmer, Stefan Luzi
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_J23105
component: BBa_B0015
component: BBa_C0040
sequence annotation: BBa_J23105
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I739001_sequence/1

BBa_B0034
type: Component
persistent identity: component2253162
display id: component2253162
version: 1
top level: BBa_I739001
definition: BBa_B0034
access: public

BBa_J23105
type: Component
persistent identity: component2253160
display id: component2253160
version: 1
top level: BBa_I739001
definition: BBa_J23105
access: public

BBa_B0015
type: Component
persistent identity: component2253173
display id: component2253173
version: 1
top level: BBa_I739001
definition: BBa_B0015
access: public

BBa_C0040
type: Component
persistent identity: component2253166
display id: component2253166
version: 1
top level: BBa_I739001
definition: tetR
access: public

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2253160
display id: annotation2253160
version: 1
top level: BBa_I739001
location: https://synbiohub.org/public/igem/BBa_I739001/annotation2253160/range2253160/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_I739001/annotation2253160/range2253160/1
type: Range
persistent identity: range2253160
display id: range2253160
version: 1
top level: BBa_I739001
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2253162
display id: annotation2253162
version: 1
top level: BBa_I739001
location: https://synbiohub.org/public/igem/BBa_I739001/annotation2253162/range2253162/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I739001/annotation2253162/range2253162/1
type: Range
persistent identity: range2253162
display id: range2253162
version: 1
top level: BBa_I739001
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2253166
display id: annotation2253166
version: 1
top level: BBa_I739001
location: https://synbiohub.org/public/igem/BBa_I739001/annotation2253166/range2253166/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I739001/annotation2253166/range2253166/1
type: Range
persistent identity: range2253166
display id: range2253166
version: 1
top level: BBa_I739001
direction: direction/reverse
start: 62
end: 746
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2253173
display id: annotation2253173
version: 1
top level: BBa_I739001
location: https://synbiohub.org/public/igem/BBa_I739001/annotation2253173/range2253173/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I739001/annotation2253173/range2253173/1
type: Range
persistent identity: range2253173
display id: range2253173
version: 1
top level: BBa_I739001
direction: direction/reverse
start: 755
end: 883
orientation: inline

https://synbiohub.org/public/igem/BBa_I729009_sequence/1
type: Sequence
persistent identity: BBa I729009 sequence
display id: BBa_I729009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K084011_sequence/1
type: Sequence
persistent identity: BBa K084011 sequence
display id: BBa_K084011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23105_sequence/1
type: Sequence
persistent identity: BBa J23105 sequence
display id: BBa_J23105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I751502_sequence/1
type: Sequence
persistent identity: BBa I751502 sequence
display id: BBa_I751502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0033_sequence/1
type: Sequence
persistent identity: BBa B0033 sequence
display id: BBa_B0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145151_sequence/1
type: Sequence
persistent identity: BBa K145151 sequence
display id: BBa_K145151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13504_sequence/1
type: Sequence
persistent identity: BBa I13504 sequence
display id: BBa_I13504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I730003_sequence/1
type: Sequence
persistent identity: BBa I730003 sequence
display id: BBa_I730003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I739001_sequence/1
type: Sequence
persistent identity: BBa I739001 sequence
display id: BBa_I739001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z