LOCUS BBa_I730007 1319 bp DNA linear 14-Nov-2024 DEFINITION pOmpRm-pTetR-LacI repressor ACCESSION BBa_I730007 VERSION BBa_I730007.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 37..44 /label=linker promoter 45..50 /label=-35 promoter 68..73 /label=-10 misc_feature 41..41 /label=A promoter 1..78 /label=BBa_R0083 misc_feature 87..105 /label=TetR 1 misc_feature 87..140 /label=BBa_R0040 promoter 106..111 /label=-35 misc_feature 112..130 /label=TetR 2 promoter 129..134 /label=-10 promoter 87..140 /label=BBa_R0040 misc_feature 153..156 /label=conserved RBS 149..160 /label=BBa_B0034 misc_feature 1256..1294 /label=LVA CDS 167..1294 /label=lacI-LVA misc_feature 167..1294 /label=BBa_C0012 misc_feature 1295..1319 /label=Help:Barcodes CDS 167..1294 /label=BBa_C0012 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgatctct tgagttggat tattctgcat 61 ttttggggag aatggactta ctagagtccc tatcagtgat agagattgac atccctatca 121 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgg tgaatgtgaa 181 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 241 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 301 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt 361 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc 421 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 481 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 541 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 601 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 661 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 721 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 781 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 841 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 901 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 961 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta 1021 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 1081 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 1141 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 1201 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc 1261 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctc //