##gff-version 3
##sequence-region BBa_I730007 1 1294
BBa_I730007	.	CDS	167	1294	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I730007	.	CDS	167	1294	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I730007	.	engineered_tag	1295	1319	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I730007	.	engineered_region	167	1294	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I730007	.	tag	1256	1294	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I730007	.	promoter	87	140	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I730007	.	engineered_region	87	140	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I730007	.	promoter	106	111	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I730007	.	non_covalent_binding_site	87	105	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I730007	.	promoter	129	134	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I730007	.	non_covalent_binding_site	112	130	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I730007	.	ribosome_entry_site	149	160	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I730007	.	conserved_region	153	156	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730007	.	promoter	1	78	.	+	0	ID=BBa_R0083;Name=BBa_R0083
BBa_I730007	.	promoter	45	50	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
BBa_I730007	.	sequence_feature	37	44	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_I730007	.	point_mutation	41	41	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_I730007	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_I730007	.	promoter	68	73	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
>BBa_I730007
tcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggactt
actagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggaga
aatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttc
ccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattac
attcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgc
acgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtaga
acgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactat
ccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgacc
agacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtca
ccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctc
actcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaa
tgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattac
cgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccg
ccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggcc
aggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgc
ctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaac
gacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc