LOCUS       BBa_I730009              852 bp    DNA     linear       22-Dec-2024
DEFINITION  pOmpRm-pLacI-TetR repressor
ACCESSION   BBa_I730009
VERSION     BBa_I730009.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    37..44
                     /label=linker
     promoter        45..50
                     /label=-35
     promoter        68..73
                     /label=-10
     misc_feature    41..41
                     /label=A
     promoter        1..78
                     /label=BBa_R0083
     misc_feature    89..105
                     /label=lac O1
     promoter        106..111
                     /label=-35
     misc_feature    112..128
                     /label=lac O1
     promoter        129..134
                     /label=-10
     misc_feature    87..140
                     /label=BBa_R0011
     promoter        87..140
                     /label=BBa_R0011
     misc_feature    154..157
                     /label=conserved
     RBS             150..161
                     /label=BBa_B0034
     CDS             171..787
                     /label=tetR
     misc_feature    788..821
                     /label=SsrA
     misc_feature    828..852
                     /label=Help:Barcodes
     CDS             168..852
                     /label=BBa_C0040
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgatctct tgagttggat tattctgcat
       61 ttttggggag aatggactta ctagagaatt gtgagcggat aacaattgac attgtgagcg
      121 gataacaaga tactgagcac atactagaga aagaggagaa atactagatg tccagattag
      181 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
      241 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
      301 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
      361 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
      421 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
      481 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
      541 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
      601 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
      661 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
      721 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
      781 gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct
      841 agtgtagatc ac
//