LOCUS       BBa_I730011             2274 bp    DNA     linear       01-Jul-2024
DEFINITION  pOmpR-ptetR-LacI repressor-YFP
ACCESSION   BBa_I730011
VERSION     BBa_I730011.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    117..135
                     /label=TetR 1
     misc_feature    117..170
                     /label=BBa_R0040
     promoter        136..141
                     /label=-35
     misc_feature    142..160
                     /label=TetR 2
     promoter        159..164
                     /label=-10
     promoter        117..170
                     /label=BBa_R0040
     misc_feature    183..186
                     /label=conserved
     RBS             179..190
                     /label=BBa_B0034
     misc_feature    1286..1324
                     /label=LVA
     CDS             197..1324
                     /label=lacI-LVA
     misc_feature    197..1324
                     /label=BBa_C0012
     misc_feature    1325..1349
                     /label=Help:Barcodes
     CDS             197..1324
                     /label=BBa_C0012
     misc_feature    1362..1365
                     /label=conserved
     RBS             1358..1369
                     /label=BBa_B0034
     misc_feature    2132..2137
                     /label=stop
     misc_feature    2093..2131
                     /label=AAV
     misc_feature    1376..2137
                     /label=BBa_E0034
     stem_loop       2157..2200
                     /label=stem_loop
     misc_feature    2146..2225
                     /label=BBa_B0010
     terminator      2146..2225
                     /label=BBa_B0010
     stem_loop       2241..2260
                     /label=T7 TE
     misc_feature    2267..2267
                     /label=stop
     polyA_site      2261..2274
                     /label=polya
     misc_feature    2234..2274
                     /label=BBa_B0012
     terminator      2234..2274
                     /label=BBa_B0012
     misc_feature    1358..2274
                     /label=BBa_E0434
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagtccc
      121 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac tactagagaa
      181 agaggagaaa tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
      241 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
      301 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
      361 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
      421 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
      481 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
      541 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
      601 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
      661 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
      721 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
      781 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
      841 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
      901 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
      961 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     1021 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     1081 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     1141 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     1201 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     1261 ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta
     1321 ataactctga tagtgctagt gtagatctct actagagaaa gaggagaaat actagatggt
     1381 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1441 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1501 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1561 gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca
     1621 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1681 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1741 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1801 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1861 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1921 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1981 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2041 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agaggcctgc
     2101 tgcaaacgac gaaaactacg ctgctgctgt ttaataatac tagagccagg catcaaataa
     2161 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2221 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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