##gff-version 3
##sequence-region BBa_I730012 1 2007
BBa_I730012	.	engineered_region	1	766	.	+	0	ID=BBa_I730002;Name=BBa_I730002
BBa_I730012	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I730002
BBa_I730012	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I730012	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I730012	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I730012	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I730012	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I730012	.	CDS	82	766	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I730002
BBa_I730012	.	engineered_tag	742	766	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I730012	.	tag	702	735	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I730012	.	CDS	85	701	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I730012	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I730002
BBa_I730012	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730012	.	engineered_region	775	2007	.	+	0	ID=BBa_I730003;Name=BBa_I730003
BBa_I730012	.	CDS	855	1982	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_I730003
BBa_I730012	.	CDS	855	1982	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I730012	.	engineered_tag	1983	2007	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I730012	.	engineered_region	855	1982	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I730012	.	tag	1944	1982	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I730012	.	ribosome_entry_site	837	848	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I730003
BBa_I730012	.	conserved_region	841	844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730012	.	promoter	775	828	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I730003
BBa_I730012	.	non_covalent_binding_site	775	793	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I730012	.	non_covalent_binding_site	800	818	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I730012	.	promoter	794	799	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I730012	.	engineered_region	775	828	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I730012	.	promoter	817	822	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_I730012
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaac
aacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctc
gacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttt
tacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcc
tacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatta
tatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaa
gggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcc
agccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaac
gacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatag
agattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgaatgtgaaa
ccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcc
acgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaaca
actggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcg
gcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgta
aagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccat
tgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatt
ttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgg
gcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgat
agcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccc
actgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtg
cggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacagga
ttttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctg
ttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgatt
cattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagc
ttaataactctgatagtgctagtgtagatctc