LOCUS       BBa_I730013             1777 bp    DNA     linear       26-Dec-2024
DEFINITION  pOmpRm-pLacI-TetR repressor-CFP
ACCESSION   BBa_I730013
VERSION     BBa_I730013.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    37..44
                     /label=linker
     promoter        45..50
                     /label=-35
     promoter        68..73
                     /label=-10
     misc_feature    41..41
                     /label=A
     promoter        1..78
                     /label=BBa_R0083
     misc_feature    89..105
                     /label=lac O1
     promoter        106..111
                     /label=-35
     misc_feature    112..128
                     /label=lac O1
     promoter        129..134
                     /label=-10
     misc_feature    87..140
                     /label=BBa_R0011
     promoter        87..140
                     /label=BBa_R0011
     misc_feature    154..157
                     /label=conserved
     RBS             150..161
                     /label=BBa_B0034
     CDS             171..787
                     /label=tetR
     misc_feature    788..821
                     /label=SsrA
     misc_feature    828..852
                     /label=Help:Barcodes
     CDS             168..852
                     /label=BBa_C0040
     misc_feature    865..868
                     /label=conserved
     RBS             861..872
                     /label=BBa_B0034
     CDS             879..1640
                     /label=CFP (LVA)
     misc_feature    1597..1634
                     /label=SsrA
     misc_feature    1635..1640
                     /label=2
     misc_feature    947..947
                     /label=A
     misc_feature    879..1640
                     /label=BBa_E0022
     misc_feature    879..1640
                     /label=BBa_E0022
     stem_loop       1660..1703
                     /label=stem_loop
     misc_feature    1649..1728
                     /label=BBa_B0010
     terminator      1649..1728
                     /label=BBa_B0010
     stem_loop       1744..1763
                     /label=T7 TE
     misc_feature    1770..1770
                     /label=stop
     polyA_site      1764..1777
                     /label=polya
     misc_feature    1737..1777
                     /label=BBa_B0012
     terminator      1737..1777
                     /label=BBa_B0012
     misc_feature    861..1777
                     /label=BBa_E0422
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgatctct tgagttggat tattctgcat
       61 ttttggggag aatggactta ctagagaatt gtgagcggat aacaattgac attgtgagcg
      121 gataacaaga tactgagcac atactagaga aagaggagaa atactagatg tccagattag
      181 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
      241 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
      301 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
      361 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
      421 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
      481 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
      541 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
      601 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
      661 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
      721 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
      781 gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct
      841 agtgtagatc actactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc
      901 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac ggccacaagt
      961 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1021 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg
     1081 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1141 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1201 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1261 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca
     1321 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1381 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1441 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1501 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1561 ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact
     1621 acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1681 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1741 actggctcac cttcgggtgg gcctttctgc gtttata
//