##gff-version 3
##sequence-region BBa_I730013 1 1777
BBa_I730013	.	engineered_region	861	1777	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_I730013	.	terminator	1649	1728	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_I730013	.	engineered_region	1649	1728	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I730013	.	stem_loop	1660	1703	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I730013	.	ribosome_entry_site	861	872	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_I730013	.	conserved_region	865	868	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730013	.	terminator	1737	1777	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_I730013	.	polyA_site	1764	1777	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I730013	.	engineered_region	1737	1777	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I730013	.	stop_codon	1770	1770	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I730013	.	stem_loop	1744	1763	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I730013	.	engineered_region	879	1640	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_I730013	.	CDS	879	1640	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I730013	.	point_mutation	947	947	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I730013	.	stop_codon	1635	1640	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I730013	.	engineered_region	879	1640	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I730013	.	tag	1597	1634	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I730013	.	promoter	1	78	.	+	0	ID=BBa_R0083;Name=BBa_R0083
BBa_I730013	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_I730013	.	promoter	68	73	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
BBa_I730013	.	point_mutation	41	41	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_I730013	.	sequence_feature	37	44	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_I730013	.	promoter	45	50	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
BBa_I730013	.	ribosome_entry_site	150	161	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I730013	.	conserved_region	154	157	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730013	.	promoter	87	140	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I730013	.	operator	112	128	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I730013	.	operator	89	105	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I730013	.	promoter	129	134	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I730013	.	engineered_region	87	140	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I730013	.	promoter	106	111	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I730013	.	CDS	168	852	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I730013	.	tag	788	821	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I730013	.	CDS	171	787	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I730013	.	engineered_tag	828	852	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_I730013
tcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggactt
actagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggag
aaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaag
gtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggc
tttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaa
gattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggta
cacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaa
tgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaa
gaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtg
cagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgc
tgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggag
aaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtga
acggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcac
caccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctacccc
gaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaagg
acgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcat
cgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgcc
gacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgacc
actaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccct
gagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatg
gacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttata