LOCUS       BBa_I730014             2244 bp    DNA     linear       24-Nov-2024
DEFINITION  pOmpRm-ptetR-LacI repressor-YFP
ACCESSION   BBa_I730014
VERSION     BBa_I730014.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    37..44
                     /label=linker
     promoter        45..50
                     /label=-35
     promoter        68..73
                     /label=-10
     misc_feature    41..41
                     /label=A
     promoter        1..78
                     /label=BBa_R0083
     misc_feature    87..105
                     /label=TetR 1
     misc_feature    87..140
                     /label=BBa_R0040
     promoter        106..111
                     /label=-35
     misc_feature    112..130
                     /label=TetR 2
     promoter        129..134
                     /label=-10
     promoter        87..140
                     /label=BBa_R0040
     misc_feature    153..156
                     /label=conserved
     RBS             149..160
                     /label=BBa_B0034
     misc_feature    1256..1294
                     /label=LVA
     CDS             167..1294
                     /label=lacI-LVA
     misc_feature    167..1294
                     /label=BBa_C0012
     misc_feature    1295..1319
                     /label=Help:Barcodes
     CDS             167..1294
                     /label=BBa_C0012
     misc_feature    1332..1335
                     /label=conserved
     RBS             1328..1339
                     /label=BBa_B0034
     misc_feature    2102..2107
                     /label=stop
     misc_feature    2063..2101
                     /label=AAV
     misc_feature    1346..2107
                     /label=BBa_E0034
     stem_loop       2127..2170
                     /label=stem_loop
     misc_feature    2116..2195
                     /label=BBa_B0010
     terminator      2116..2195
                     /label=BBa_B0010
     stem_loop       2211..2230
                     /label=T7 TE
     misc_feature    2237..2237
                     /label=stop
     polyA_site      2231..2244
                     /label=polya
     misc_feature    2204..2244
                     /label=BBa_B0012
     terminator      2204..2244
                     /label=BBa_B0012
     misc_feature    1328..2244
                     /label=BBa_E0434
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgatctct tgagttggat tattctgcat
       61 ttttggggag aatggactta ctagagtccc tatcagtgat agagattgac atccctatca
      121 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgg tgaatgtgaa
      181 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
      241 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
      301 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
      361 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
      421 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
      481 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
      541 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
      601 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
      661 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
      721 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
      781 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
      841 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
      901 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
      961 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     1021 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     1081 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     1141 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     1201 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc
     1261 aaacgacgaa aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct
     1321 actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt
     1381 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1441 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1501 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt
     1561 cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg
     1621 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1681 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1741 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1801 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1861 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1921 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     1981 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2041 cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctgctgctgt
     2101 ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2161 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2221 cgggtgggcc tttctgcgtt tata
//