LOCUS BBa_I730015 4059 bp DNA linear 22-Dec-2024 DEFINITION Promoter calibrator device ACCESSION BBa_I730015 VERSION BBa_I730015.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 119..135 /label=lac O1 promoter 136..141 /label=-35 misc_feature 142..158 /label=lac O1 promoter 159..164 /label=-10 misc_feature 117..170 /label=BBa_R0011 promoter 117..170 /label=BBa_R0011 misc_feature 184..187 /label=conserved RBS 180..191 /label=BBa_B0034 CDS 201..817 /label=tetR misc_feature 818..851 /label=SsrA misc_feature 858..882 /label=Help:Barcodes CDS 198..882 /label=BBa_C0040 misc_feature 895..898 /label=conserved RBS 891..902 /label=BBa_B0034 CDS 909..1670 /label=CFP (LVA) misc_feature 1627..1664 /label=SsrA misc_feature 1665..1670 /label=2 misc_feature 977..977 /label=A misc_feature 909..1670 /label=BBa_E0022 misc_feature 909..1670 /label=BBa_E0022 stem_loop 1690..1733 /label=stem_loop misc_feature 1679..1758 /label=BBa_B0010 terminator 1679..1758 /label=BBa_B0010 stem_loop 1774..1793 /label=T7 TE misc_feature 1800..1800 /label=stop polyA_site 1794..1807 /label=polya misc_feature 1767..1807 /label=BBa_B0012 terminator 1767..1807 /label=BBa_B0012 misc_feature 891..1807 /label=BBa_E0422 misc_feature 1..1807 /label=BBa_I730010 misc_feature 1828..1845 /label=C1 OmpR misc_feature 1852..1859 /label=linker promoter 1860..1865 /label=-35 promoter 1883..1888 /label=-10 misc_feature 1856..1856 /label=A promoter 1816..1893 /label=BBa_R0083 misc_feature 1902..1920 /label=TetR 1 misc_feature 1902..1955 /label=BBa_R0040 promoter 1921..1926 /label=-35 misc_feature 1927..1945 /label=TetR 2 promoter 1944..1949 /label=-10 promoter 1902..1955 /label=BBa_R0040 misc_feature 1968..1971 /label=conserved RBS 1964..1975 /label=BBa_B0034 misc_feature 3071..3109 /label=LVA CDS 1982..3109 /label=lacI-LVA misc_feature 1982..3109 /label=BBa_C0012 misc_feature 3110..3134 /label=Help:Barcodes CDS 1982..3109 /label=BBa_C0012 misc_feature 3147..3150 /label=conserved RBS 3143..3154 /label=BBa_B0034 misc_feature 3917..3922 /label=stop misc_feature 3878..3916 /label=AAV misc_feature 3161..3922 /label=BBa_E0034 stem_loop 3942..3985 /label=stem_loop misc_feature 3931..4010 /label=BBa_B0010 terminator 3931..4010 /label=BBa_B0010 stem_loop 4026..4045 /label=T7 TE misc_feature 4052..4052 /label=stop polyA_site 4046..4059 /label=polya misc_feature 4019..4059 /label=BBa_B0012 terminator 4019..4059 /label=BBa_B0012 misc_feature 3143..4059 /label=BBa_E0434 misc_feature 1816..4059 /label=BBa_I730014 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaatt 121 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga 181 aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac agcgcattag 241 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag 301 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct 361 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct 421 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg 481 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa 541 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca 601 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta 661 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg 721 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat 781 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg 841 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag aaagaggaga 901 aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 961 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 1021 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 1081 ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc taccccgacc 1141 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 1201 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 1261 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 1321 tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc gccgacaagc 1381 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 1441 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 1501 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 1561 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 1621 acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc 1681 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1741 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1801 gtttatatac tagagtccct tgcatttaca ttttgaaaca tctatagcga tctcttgagt 1861 tggattattc tgcatttttg gggagaatgg acttactaga gtccctatca gtgatagaga 1921 ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta 1981 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 2041 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 2101 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 2161 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 2221 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 2281 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 2341 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 2401 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 2461 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 2521 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 2581 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 2641 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 2701 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 2761 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 2821 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 2881 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 2941 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 3001 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 3061 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 3121 ctagtgtaga tctctactag agaaagagga gaaatactag atggtgagca agggcgagga 3181 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 3241 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 3301 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta 3361 cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc 3421 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 3481 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 3541 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 3601 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 3661 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 3721 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc 3781 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 3841 cgccgggatc actctcggca tggacgagct gtacaagagg cctgctgcaa acgacgaaaa 3901 ctacgctgct gctgtttaat aatactagag ccaggcatca aataaaacga aaggctcagt 3961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 4021 acactggctc accttcgggt gggcctttct gcgtttata //