##gff-version 3
##sequence-region BBa_I730015 1 4059
BBa_I730015	.	engineered_region	1816	4059	.	+	0	ID=BBa_I730014;Name=BBa_I730014
BBa_I730015	.	promoter	1816	1893	.	+	0	ID=BBa_R0083;Name=BBa_R0083;Parent=BBa_I730014
BBa_I730015	.	point_mutation	1856	1856	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_I730015	.	promoter	1860	1865	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
BBa_I730015	.	sequence_feature	1852	1859	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_I730015	.	promoter	1883	1888	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
BBa_I730015	.	non_covalent_binding_site	1828	1845	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_I730015	.	promoter	1902	1955	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I730014
BBa_I730015	.	promoter	1921	1926	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I730015	.	non_covalent_binding_site	1927	1945	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I730015	.	engineered_region	1902	1955	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I730015	.	promoter	1944	1949	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I730015	.	non_covalent_binding_site	1902	1920	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I730015	.	engineered_region	3143	4059	.	+	0	ID=BBa_E0434;Name=BBa_E0434;Parent=BBa_I730014
BBa_I730015	.	terminator	3931	4010	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0434
BBa_I730015	.	engineered_region	3931	4010	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I730015	.	stem_loop	3942	3985	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I730015	.	engineered_region	3161	3922	.	+	0	ID=BBa_E0034;Name=BBa_E0034;Parent=BBa_E0434
BBa_I730015	.	stop_codon	3917	3922	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I730015	.	tag	3878	3916	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I730015	.	ribosome_entry_site	3143	3154	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0434
BBa_I730015	.	conserved_region	3147	3150	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730015	.	terminator	4019	4059	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0434
BBa_I730015	.	engineered_region	4019	4059	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I730015	.	stop_codon	4052	4052	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I730015	.	stem_loop	4026	4045	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I730015	.	polyA_site	4046	4059	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I730015	.	ribosome_entry_site	1964	1975	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I730014
BBa_I730015	.	conserved_region	1968	1971	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730015	.	CDS	1982	3109	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_I730014
BBa_I730015	.	engineered_tag	3110	3134	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I730015	.	engineered_region	1982	3109	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I730015	.	CDS	1982	3109	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I730015	.	tag	3071	3109	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I730015	.	engineered_region	1	1807	.	+	0	ID=BBa_I730010;Name=BBa_I730010
BBa_I730015	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_I730010
BBa_I730015	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_I730015	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_I730015	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_I730015	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_I730015	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_I730015	.	engineered_region	891	1807	.	+	0	ID=BBa_E0422;Name=BBa_E0422;Parent=BBa_I730010
BBa_I730015	.	terminator	1679	1758	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_I730015	.	engineered_region	1679	1758	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I730015	.	stem_loop	1690	1733	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I730015	.	terminator	1767	1807	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_I730015	.	engineered_region	1767	1807	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I730015	.	stop_codon	1800	1800	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I730015	.	stem_loop	1774	1793	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I730015	.	polyA_site	1794	1807	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I730015	.	engineered_region	909	1670	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_I730015	.	CDS	909	1670	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I730015	.	tag	1627	1664	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I730015	.	point_mutation	977	977	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I730015	.	stop_codon	1665	1670	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I730015	.	engineered_region	909	1670	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I730015	.	ribosome_entry_site	891	902	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_I730015	.	conserved_region	895	898	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I730015	.	CDS	198	882	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I730010
BBa_I730015	.	engineered_tag	858	882	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I730015	.	tag	818	851	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I730015	.	CDS	201	817	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I730015	.	promoter	117	170	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I730010
BBa_I730015	.	promoter	159	164	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I730015	.	promoter	136	141	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I730015	.	operator	142	158	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I730015	.	engineered_region	117	170	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I730015	.	operator	119	135	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I730015	.	ribosome_entry_site	180	191	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I730010
BBa_I730015	.	conserved_region	184	187	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I730015
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaattgtgagcggataacaattgacattgtgagcggataacaa
gatactgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcat
tagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctac
attgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcac
ttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtc
atcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctt
tttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgta
ttggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgac
aagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattaga
aaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgc
tagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgc
ccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccaccta
cggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacc
tggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggct
acgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacac
cctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaaca
tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga
caaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttc
gtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttag
tagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtc
ccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggacttac
tagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
tactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttccc
gcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacat
tcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcac
gcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaac
gaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatcc
gctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccag
acacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcacc
agcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcac
tcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatg
ctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccg
agtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgcc
gttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccag
gcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcct
ctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacga
cgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactag
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccaca
agttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaa
gctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatg
aagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggca
actacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaa
ggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcag
aagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagc
agaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaaga
ccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctg
tacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata