LOCUS BBa_I731005 2813 bp DNA linear 27-Dec-2024 DEFINITION Constitutive lux I + Constitutive lux R + luxpR-regulated GFP ACCESSION BBa_I731005 VERSION BBa_I731005.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 660..691 /label=LVA CDS 81..659 /label=luxI misc_feature 81..698 /label=BBa_C0061 misc_feature 699..723 /label=Help:Barcodes CDS 81..698 /label=BBa_C0061 stem_loop 743..786 /label=stem_loop misc_feature 732..811 /label=BBa_B0010 terminator 732..811 /label=BBa_B0010 stem_loop 827..846 /label=T7 TE misc_feature 853..853 /label=stop polyA_site 847..860 /label=polya misc_feature 820..860 /label=BBa_B0012 terminator 820..860 /label=BBa_B0012 misc_feature 63..860 /label=BBa_F1610 misc_feature 1..860 /label=BBa_J23039 misc_feature 869..887 /label=TetR 1 misc_feature 869..922 /label=BBa_R0040 promoter 888..893 /label=-35 misc_feature 894..912 /label=TetR 2 promoter 911..916 /label=-10 promoter 869..922 /label=BBa_R0040 misc_feature 935..938 /label=conserved RBS 931..942 /label=BBa_B0034 misc_feature 949..950 /label=prefix misc_feature 1122..1122 /label=T misc_feature 1440..1440 /label=A CDS 949..1698 /label=luxR misc_feature 949..1704 /label=BBa_C0062 misc_feature 1705..1729 /label=Help:Barcodes CDS 949..1704 /label=BBa_C0062 stem_loop 1749..1792 /label=stem_loop misc_feature 1738..1817 /label=BBa_B0010 terminator 1738..1817 /label=BBa_B0010 stem_loop 1833..1852 /label=T7 TE misc_feature 1859..1859 /label=stop polyA_site 1853..1866 /label=polya misc_feature 1826..1866 /label=BBa_B0012 terminator 1826..1866 /label=BBa_B0012 misc_feature 1875..1894 /label=LuxR/HSL promoter 1894..1899 /label=-35 promoter 1916..1921 /label=-10 misc_feature 1927..1927 /label=start misc_feature 1875..1929 /label=BBa_R0062 promoter 1875..1929 /label=BBa_R0062 RBS 1938..1950 /label=RBS-3\Weak RBS 1944..1947 /label=RBS misc_feature 1938..1950 /label=BBa_B0032 RBS 1938..1950 /label=BBa_B0032 CDS 1957..2676 /label=GFP protein CDS 1957..2676 /label=BBa_E0040 stem_loop 2696..2739 /label=stem_loop misc_feature 2685..2764 /label=BBa_B0010 terminator 2685..2764 /label=BBa_B0010 stem_loop 2780..2799 /label=T7 TE misc_feature 2806..2806 /label=stop polyA_site 2800..2813 /label=polya misc_feature 2773..2813 /label=BBa_B0012 terminator 2773..2813 /label=BBa_B0012 misc_feature 869..2813 /label=BBa_T9002 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc 121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg 181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag 241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta 301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc 361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga 421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg 481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa 541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata 601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg 661 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat 721 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 781 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 841 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat 901 cagtgataga gatactgagc actactagag aaagaggaga aatactagat gaaaaacata 961 aatgccgacg acacatacag aataattaat aaaattaaag cttgtagaag caataatgat 1021 attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg 1081 atcatttatc ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa 1141 aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat 1201 tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa 1261 aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc 1321 cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac 1381 aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct 1441 ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc 1501 aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca 1561 aaaatattag gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa 1621 ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat 1681 tgcccatact ttaaaaatta ataacactga tagtgctagt gtagatcact actagagcca 1741 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 1801 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 1861 tttatatact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag 1921 tcgaataaat actagagtca cacaggaaag tactagatgc gtaaaggaga agaacttttc 1981 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 2041 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 2101 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt 2161 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 2221 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 2281 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 2341 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 2401 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 2461 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 2521 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 2581 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 2641 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa 2701 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 2761 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //