##gff-version 3
##sequence-region BBa_I731005 1 2813
BBa_I731005	.	engineered_region	1	860	.	+	0	ID=BBa_J23039;Name=BBa_J23039
BBa_I731005	.	engineered_region	63	860	.	+	0	ID=BBa_F1610;Name=BBa_F1610;Parent=BBa_J23039
BBa_I731005	.	CDS	81	698	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_I731005	.	tag	660	691	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I731005	.	engineered_tag	699	723	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I731005	.	engineered_region	81	698	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I731005	.	CDS	81	659	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I731005	.	terminator	732	811	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_I731005	.	engineered_region	732	811	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I731005	.	stem_loop	743	786	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I731005	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_I731005	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I731005	.	terminator	820	860	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_I731005	.	stem_loop	827	846	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I731005	.	engineered_region	820	860	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I731005	.	stop_codon	853	853	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I731005	.	polyA_site	847	860	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I731005	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J23039
BBa_I731005	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I731005	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I731005	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I731005	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I731005	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I731005	.	engineered_region	869	2813	.	+	0	ID=BBa_T9002;Name=BBa_T9002
BBa_I731005	.	terminator	1826	1866	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_I731005	.	stem_loop	1833	1852	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I731005	.	engineered_region	1826	1866	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I731005	.	stop_codon	1859	1859	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I731005	.	polyA_site	1853	1866	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I731005	.	CDS	949	1704	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_T9002
BBa_I731005	.	engineered_tag	1705	1729	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I731005	.	engineered_region	949	950	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I731005	.	CDS	949	1698	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I731005	.	point_mutation	1122	1122	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I731005	.	engineered_region	949	1704	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I731005	.	point_mutation	1440	1440	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I731005	.	ribosome_entry_site	931	942	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_T9002
BBa_I731005	.	conserved_region	935	938	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I731005	.	ribosome_entry_site	1938	1950	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_T9002
BBa_I731005	.	ribosome_entry_site	1938	1950	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I731005	.	ribosome_entry_site	1944	1947	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I731005	.	engineered_region	1938	1950	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I731005	.	promoter	1875	1929	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_T9002
BBa_I731005	.	non_covalent_binding_site	1875	1894	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I731005	.	promoter	1894	1899	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I731005	.	engineered_region	1875	1929	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I731005	.	promoter	1916	1921	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I731005	.	start_codon	1927	1927	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I731005	.	terminator	2685	2764	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_I731005	.	engineered_region	2685	2764	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I731005	.	stem_loop	2696	2739	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I731005	.	terminator	1738	1817	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_T9002
BBa_I731005	.	engineered_region	1738	1817	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I731005	.	stem_loop	1749	1792	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I731005	.	promoter	869	922	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_T9002
BBa_I731005	.	promoter	888	893	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I731005	.	non_covalent_binding_site	869	887	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I731005	.	engineered_region	869	922	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I731005	.	promoter	911	916	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I731005	.	non_covalent_binding_site	894	912	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I731005	.	terminator	2773	2813	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_T9002
BBa_I731005	.	stem_loop	2780	2799	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I731005	.	engineered_region	2773	2813	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I731005	.	stop_codon	2806	2806	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I731005	.	polyA_site	2800	2813	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I731005	.	CDS	1957	2676	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_T9002
BBa_I731005	.	CDS	1957	2676	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_I731005
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttat
caagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatg
cagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatat
gctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtttt
gctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaac
acgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagt
tccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaat
gaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagt
ccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactag
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatc
aatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaa
atctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgat
cctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaat
ctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatgg
cttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacata
ccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacca
aaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtga
gcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagca
attttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttt
acgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaac
ttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagaggg
tgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaaca
cttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaaga
gtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagt
caagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgga
cacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaact
tcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatgg
ccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccac
atggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata