LOCUS       BBa_I731006             1592 bp    DNA     linear       09-Dec-2025
DEFINITION  Constitutive lux I + mRFP1
ACCESSION   BBa_I731006
VERSION     BBa_I731006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    660..691
                     /label=LVA
     CDS             81..659
                     /label=luxI
     misc_feature    81..698
                     /label=BBa_C0061
     misc_feature    699..723
                     /label=Help:Barcodes
     CDS             81..698
                     /label=BBa_C0061
     misc_feature    63..723
                     /label=BBa_C0261
     misc_feature    1..723
                     /label=BBa_S03623
     misc_feature    736..739
                     /label=conserved
     RBS             732..743
                     /label=BBa_B0034
     CDS             750..1424
                     /label=mrfp1
     misc_feature    1431..1455
                     /label=Help:Barcodes
     CDS             750..1430
                     /label=BBa_E1010
     stem_loop       1475..1518
                     /label=stem_loop
     misc_feature    1464..1543
                     /label=BBa_B0010
     terminator      1464..1543
                     /label=BBa_B0010
     stem_loop       1559..1578
                     /label=T7 TE
     misc_feature    1585..1585
                     /label=stop
     polyA_site      1579..1592
                     /label=polya
     misc_feature    1552..1592
                     /label=BBa_B0012
     terminator      1552..1592
                     /label=BBa_B0012
     misc_feature    732..1592
                     /label=BBa_I13507
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc
      121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg
      181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag
      241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta
      301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc
      361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga
      421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg
      481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa
      541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata
      601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg
      661 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat
      721 ctctactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
      781 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
      841 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
      901 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
      961 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     1021 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
     1081 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     1141 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     1201 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     1261 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     1321 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
     1381 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt
     1441 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1501 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1561 ctcaccttcg ggtgggcctt tctgcgttta ta
//