LOCUS       BBa_I731007             1593 bp    DNA     linear       30-Jun-2024
DEFINITION  lacI+pL-promoter regulated lux I + mRFP1
ACCESSION   BBa_I731007
VERSION     BBa_I731007.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    661..692
                     /label=LVA
     CDS             82..660
                     /label=luxI
     misc_feature    82..699
                     /label=BBa_C0061
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             82..699
                     /label=BBa_C0061
     misc_feature    64..724
                     /label=BBa_C0261
     misc_feature    1..724
                     /label=BBa_S03608
     misc_feature    737..740
                     /label=conserved
     RBS             733..744
                     /label=BBa_B0034
     CDS             751..1425
                     /label=mrfp1
     misc_feature    1432..1456
                     /label=Help:Barcodes
     CDS             751..1431
                     /label=BBa_E1010
     stem_loop       1476..1519
                     /label=stem_loop
     misc_feature    1465..1544
                     /label=BBa_B0010
     terminator      1465..1544
                     /label=BBa_B0010
     stem_loop       1560..1579
                     /label=T7 TE
     misc_feature    1586..1586
                     /label=stop
     polyA_site      1580..1593
                     /label=polya
     misc_feature    1553..1593
                     /label=BBa_B0012
     terminator      1553..1593
                     /label=BBa_B0012
     misc_feature    733..1593
                     /label=BBa_I13507
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      721 tctctactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag
      781 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
      841 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
      901 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
      961 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
     1021 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
     1081 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
     1141 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
     1201 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
     1261 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
     1321 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     1381 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
     1441 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1501 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1561 gctcaccttc gggtgggcct ttctgcgttt ata
//