LOCUS       BBa_I731009             1607 bp    DNA     linear       15-Jun-2024
DEFINITION  Constitutive lux I + GFP
ACCESSION   BBa_I731009
VERSION     BBa_I731009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    660..691
                     /label=LVA
     CDS             81..659
                     /label=luxI
     misc_feature    81..698
                     /label=BBa_C0061
     misc_feature    699..723
                     /label=Help:Barcodes
     CDS             81..698
                     /label=BBa_C0061
     misc_feature    63..723
                     /label=BBa_C0261
     misc_feature    1..723
                     /label=BBa_S03623
     RBS             732..744
                     /label=RBS-3\Weak
     RBS             738..741
                     /label=RBS
     misc_feature    732..744
                     /label=BBa_B0032
     RBS             732..744
                     /label=BBa_B0032
     CDS             751..1470
                     /label=GFP protein
     CDS             751..1470
                     /label=BBa_E0040
     stem_loop       1490..1533
                     /label=stem_loop
     misc_feature    1479..1558
                     /label=BBa_B0010
     terminator      1479..1558
                     /label=BBa_B0010
     stem_loop       1574..1593
                     /label=T7 TE
     misc_feature    1600..1600
                     /label=stop
     polyA_site      1594..1607
                     /label=polya
     misc_feature    1567..1607
                     /label=BBa_B0012
     terminator      1567..1607
                     /label=BBa_B0012
     misc_feature    732..1607
                     /label=BBa_E0240
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc
      121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg
      181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag
      241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta
      301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc
      361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga
      421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg
      481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa
      541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata
      601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg
      661 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat
      721 ctctactaga gtcacacagg aaagtactag atgcgtaaag gagaagaact tttcactgga
      781 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      841 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      901 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      961 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     1021 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     1081 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     1141 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     1201 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     1261 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     1321 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     1381 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     1441 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     1501 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1561 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//