LOCUS       BBa_I731010             1608 bp    DNA     linear       30-Jun-2024
DEFINITION  lacI+pl-promoter regulated lux I + GFP
ACCESSION   BBa_I731010
VERSION     BBa_I731010.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    661..692
                     /label=LVA
     CDS             82..660
                     /label=luxI
     misc_feature    82..699
                     /label=BBa_C0061
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             82..699
                     /label=BBa_C0061
     misc_feature    64..724
                     /label=BBa_C0261
     misc_feature    1..724
                     /label=BBa_S03608
     RBS             733..745
                     /label=RBS-3\Weak
     RBS             739..742
                     /label=RBS
     misc_feature    733..745
                     /label=BBa_B0032
     RBS             733..745
                     /label=BBa_B0032
     CDS             752..1471
                     /label=GFP protein
     CDS             752..1471
                     /label=BBa_E0040
     stem_loop       1491..1534
                     /label=stem_loop
     misc_feature    1480..1559
                     /label=BBa_B0010
     terminator      1480..1559
                     /label=BBa_B0010
     stem_loop       1575..1594
                     /label=T7 TE
     misc_feature    1601..1601
                     /label=stop
     polyA_site      1595..1608
                     /label=polya
     misc_feature    1568..1608
                     /label=BBa_B0012
     terminator      1568..1608
                     /label=BBa_B0012
     misc_feature    733..1608
                     /label=BBa_E0240
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      721 tctctactag agtcacacag gaaagtacta gatgcgtaaa ggagaagaac ttttcactgg
      781 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      841 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      901 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
      961 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     1021 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     1081 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
     1141 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     1201 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     1261 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1321 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     1381 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     1441 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
     1501 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1561 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//