##gff-version 3
##sequence-region BBa_I731010 1 1608
BBa_I731010	.	engineered_region	1	724	.	+	0	ID=BBa_S03608;Name=BBa_S03608
BBa_I731010	.	engineered_region	64	724	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_S03608
BBa_I731010	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_I731010	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I731010	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_I731010	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I731010	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I731010	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I731010	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I731010	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_S03608
BBa_I731010	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I731010	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I731010	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I731010	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I731010	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I731010	.	engineered_region	733	1608	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_I731010	.	terminator	1568	1608	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_I731010	.	engineered_region	1568	1608	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I731010	.	stem_loop	1575	1594	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I731010	.	stop_codon	1601	1601	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I731010	.	polyA_site	1595	1608	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I731010	.	terminator	1480	1559	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_I731010	.	engineered_region	1480	1559	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I731010	.	stem_loop	1491	1534	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I731010	.	ribosome_entry_site	733	745	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_I731010	.	ribosome_entry_site	733	745	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I731010	.	ribosome_entry_site	739	742	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I731010	.	engineered_region	733	745	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I731010	.	CDS	752	1471	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_I731010	.	CDS	752	1471	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_I731010
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtccca
attcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacg
gaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtta
tggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttat
gtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccc
ttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaacta
taactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacatt
gaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagaca
accattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgt
aacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata