LOCUS       BBa_I731013             1946 bp    DNA     linear       26-Dec-2024
DEFINITION  luxpR-regulated luxR + luxpR-regulated GFP
ACCESSION   BBa_I731013
VERSION     BBa_I731013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    64..1062
                     /label=BBa_A340620
     RBS             1071..1083
                     /label=RBS-3\Weak
     RBS             1077..1080
                     /label=RBS
     misc_feature    1071..1083
                     /label=BBa_B0032
     RBS             1071..1083
                     /label=BBa_B0032
     CDS             1090..1809
                     /label=GFP protein
     CDS             1090..1809
                     /label=BBa_E0040
     stem_loop       1829..1872
                     /label=stem_loop
     misc_feature    1818..1897
                     /label=BBa_B0010
     terminator      1818..1897
                     /label=BBa_B0010
     stem_loop       1913..1932
                     /label=T7 TE
     misc_feature    1939..1939
                     /label=stop
     polyA_site      1933..1946
                     /label=polya
     misc_feature    1906..1946
                     /label=BBa_B0012
     terminator      1906..1946
                     /label=BBa_B0012
     misc_feature    1071..1946
                     /label=BBa_E0240
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga
     1081 aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     1141 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     1201 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     1261 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
     1321 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     1381 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     1441 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     1501 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     1561 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1621 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1681 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1741 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1801 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1921 ttcgggtggg cctttctgcg tttata
//