LOCUS BBa_I731015 2615 bp DNA linear 15-Dec-2024 DEFINITION luxpR controlled 3OC6HSL sender + receiver ACCESSION BBa_I731015 VERSION BBa_I731015.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 64..1062 /label=BBa_A340620 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 misc_feature 1668..1699 /label=LVA CDS 1089..1667 /label=luxI misc_feature 1089..1706 /label=BBa_C0061 misc_feature 1707..1731 /label=Help:Barcodes CDS 1089..1706 /label=BBa_C0061 misc_feature 1071..1731 /label=BBa_C0261 RBS 1740..1752 /label=RBS-3\Weak RBS 1746..1749 /label=RBS misc_feature 1740..1752 /label=BBa_B0032 RBS 1740..1752 /label=BBa_B0032 CDS 1759..2478 /label=GFP protein CDS 1759..2478 /label=BBa_E0040 stem_loop 2498..2541 /label=stem_loop misc_feature 2487..2566 /label=BBa_B0010 terminator 2487..2566 /label=BBa_B0010 stem_loop 2582..2601 /label=T7 TE misc_feature 2608..2608 /label=stop polyA_site 2602..2615 /label=polya misc_feature 2575..2615 /label=BBa_B0012 terminator 2575..2615 /label=BBa_B0012 misc_feature 1740..2615 /label=BBa_E0240 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca tcggaggagt 1141 ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag tgggacttag 1201 ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa tatatttatg 1261 cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca acaggtgatt 1321 atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc aaagatccta 1381 atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata aataactctg 1441 ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt agtcaaggta 1501 ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag cgtattaaag 1561 ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact aaatcggttg 1621 tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct gcaaacgacg 1681 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct ctactagagt 1741 cacacaggaa agtactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 1801 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 1861 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 1921 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 1981 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 2041 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 2101 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 2161 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 2221 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 2281 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 2341 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 2401 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 2461 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2521 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2581 tggctcacct tcgggtgggc ctttctgcgt ttata //