LOCUS       BBa_I731017             1600 bp    DNA     linear       27-Dec-2024
DEFINITION  Ptet bicistronic 3OC6HSL Sender + mCherry
ACCESSION   BBa_I731017
VERSION     BBa_I731017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    660..691
                     /label=LVA
     CDS             81..659
                     /label=luxI
     misc_feature    81..698
                     /label=BBa_C0061
     misc_feature    699..723
                     /label=Help:Barcodes
     CDS             81..698
                     /label=BBa_C0061
     misc_feature    63..723
                     /label=BBa_C0261
     misc_feature    1..723
                     /label=BBa_S03623
     misc_feature    736..739
                     /label=conserved
     RBS             732..743
                     /label=BBa_B0034
     CDS             750..1460
                     /label=mCherry
     misc_feature    1101..1101
                     /label=C->T (removing PstI site)
     misc_feature    750..1463
                     /label=BBa_J06504
     stem_loop       1483..1526
                     /label=stem_loop
     misc_feature    1472..1551
                     /label=BBa_B0010
     terminator      1472..1551
                     /label=BBa_B0010
     stem_loop       1567..1586
                     /label=T7 TE
     misc_feature    1593..1593
                     /label=stop
     polyA_site      1587..1600
                     /label=polya
     misc_feature    1560..1600
                     /label=BBa_B0012
     terminator      1560..1600
                     /label=BBa_B0012
     misc_feature    732..1600
                     /label=BBa_J06702
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc
      121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg
      181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag
      241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta
      301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc
      361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga
      421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg
      481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa
      541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata
      601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg
      661 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat
      721 ctctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag gataacatgg
      781 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      841 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      901 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      961 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1021 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1081 ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1141 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1201 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1261 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1321 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1381 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1441 tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1501 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1561 cacactggct caccttcggg tgggcctttc tgcgtttata
//