LOCUS       BBa_I732004             2751 bp    DNA     linear       25-Nov-2024
DEFINITION  Over-express gyrA
ACCESSION   BBa_I732004
VERSION     BBa_I732004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     RBS             46..60
                     /label=RBS-1\Strong
     RBS             53..57
                     /label=RBS
     misc_feature    46..60
                     /label=BBa_B0030
     RBS             46..60
                     /label=BBa_B0030
     CDS             67..2697
                     /label=gyrA
     misc_feature    1275..1275
                     /label=T
     CDS             67..2697
                     /label=BBa_I732003
     stem_loop       2718..2740
                     /label=stem_loop
     misc_feature    2706..2751
                     /label=BBa_B0011
     terminator      2706..2751
                     /label=BBa_B0011
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagattaa agaggagaaa
       61 tactagatga gcgaccttgc gagagaaatt acaccggtca acattgagga agagctgaag
      121 agctcctatc tggattatgc gatgtcggtc attgttggcc gtgcgctgcc agatgtccga
      181 gatggcctga agccggtaca ccgtcgcgta ctttacgcca tgaacgtact aggcaatgac
      241 tggaacaaag cctataaaaa atctgcccgt gtcgttggtg acgtaatcgg taaataccat
      301 ccccatggtg actcggcggt ctatgacacg atcgtccgca tggcgcagcc attctcgctg
      361 cgttatatgc tggtagacgg tcagggtaac ttcggttcta tcgacggcga ctctgcggcg
      421 gcaatgcgtt atacggaaat ccgtctggcg aaaattgccc atgaactgat ggccgatctc
      481 gaaaaagaga cggtcgattt cgttgataac tatgacggca cggaaaaaat tccggacgtc
      541 atgccaacca aaattcctaa cctgctggtg aacggttctt ccggtatcgc cgtaggtatg
      601 gcaaccaaca tcccgccgca caacctgacg gaagtcatca acggttgtct ggcgtatatt
      661 gatgatgaag acatcagcat tgaagggctg atggaacaca tcccggggcc ggacttcccg
      721 acggcggcaa tcattaacgg tcgtcgcggt attgaagaag cttaccgtac cggtcgcggc
      781 aaggtgtata tccgcgctcg cgcagaagtg gaagttgacg ccaaaaccgg tcgtgaaacc
      841 attatcgtcc acgaaattcc gtatcaggta aacaaagcgc gcctgatcga gaagattgcg
      901 gaactggtaa aagaaaaacg cgtggaaggc atcagcgcgc tgcgtgacga gtctgacaaa
      961 gacggtatgc gcatcgtgat tgaagtgaaa cgcgatgcgg tcggtgaagt tgtgctcaac
     1021 aacctctact cccagaccca gttgcaggtt tctttcggta tcaacatggt ggcattgcac
     1081 catggtcagc cgaagatcat gaacctgaaa gacatcatcg cggcgtttgt tcgtcaccgc
     1141 cgtgaagtgg tgacccgtcg tactattttc gaactgcgta aagctcgcga tcgtgctcat
     1201 atccttgaag cattagccgt ggcgctggcg aacatcgacc cgatcatcga actgatccgt
     1261 catgcgccga cgccagcaga agcgaaaact gcgctggttg ctaatccgtg gcagctgggc
     1321 aacgttgccg cgatgctcga acgtgctggc gacgatgctg cgcgtccgga atggctggag
     1381 ccagagttcg gcgtgcgtga tggtctgtac tacctgaccg aacagcaagc tcaggcgatt
     1441 ctggatctgc gtttgcagaa actgaccggt cttgagcacg aaaaactgct cgacgaatac
     1501 aaagagctgc tggatcagat cgcggaactg ttgcgtattc ttggtagcgc cgatcgtctg
     1561 atggaagtga tccgtgaaga gctggagctg gttcgtgaac agttcggtga caaacgtcgt
     1621 actgaaatca ccgccaacag cgcagacatc aacctggaag atctgatcac ccaggaagat
     1681 gtggtcgtga cgctctctca ccagggctac gttaagtatc agccgctttc tgaatacgaa
     1741 gcgcagcgtc gtggcgggaa aggtaaatct gccgcacgta ttaaagaaga agactttatc
     1801 gaccgactgc tggtggcgaa cactcacgac catattctgt gcttctccag ccgtggtcgc
     1861 gtctattcga tgaaagttta tcagttgccg gaagccactc gtggcgcgcg cggtcgtccg
     1921 atcgtcaacc tgctgccgct ggagcaggac gaacgtatca ctgcgatcct gccagtgacc
     1981 gagtttgaag aaggcgtgaa agtcttcatg gcgaccgcta acggtaccgt gaagaaaact
     2041 gtcctcaccg agttcaaccg tctgcgtacc gccggtaaag tggcgatcaa actggttgac
     2101 ggcgatgagc tgatcggcgt tgacctgacc agcggcgaag acgaagtaat gctgttctcc
     2161 gctgaaggta aagtggtgcg ctttaaagag tcttctgtcc gtgcgatggg ctgcaacacc
     2221 accggtgttc gcggtattcg cttaggtgaa ggcgataaag tcgtctctct gatcgtgcct
     2281 cgtggcgatg gcgcaatcct caccgcaacg caaaacggtt acggtaaacg taccgcagtg
     2341 gcggaatacc caaccaagtc gcgtgcgacg aaaggggtta tctccatcaa ggttaccgaa
     2401 cgtaacggtt tagttgttgg cgcggtacag gtagatgact gcgaccagat catgatgatc
     2461 accgatgccg gtacgctggt acgtactcgc gtttcggaaa tcagcatcgt gggccgtaac
     2521 acccagggcg tgatcctcat ccgtactgcg gaagatgaaa acgtagtggg tctgcaacgt
     2581 gttgctgaac cggttgacga ggaagatctg gataccatcg acggcagtgc cgcggaaggg
     2641 gacgatgaaa tcgctccgga agtggacgtt gacgacgagc cagaagaaga ataataatac
     2701 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt t
//