LOCUS       BBa_I732010             2926 bp    DNA     linear       22-Nov-2024
DEFINITION  S03600.S03520.E0840
ACCESSION   BBa_I732010
VERSION     BBa_I732010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     misc_feature    874..877
                     /label=conserved
     RBS             870..881
                     /label=BBa_B0034
     misc_feature    1977..2015
                     /label=LVA
     CDS             888..2015
                     /label=lacI-LVA
     misc_feature    888..2015
                     /label=BBa_C0012
     misc_feature    2016..2040
                     /label=Help:Barcodes
     CDS             888..2015
                     /label=BBa_C0012
     RBS             2049..2063
                     /label=RBS-1\Strong
     RBS             2056..2060
                     /label=RBS
     misc_feature    2049..2063
                     /label=BBa_B0030
     RBS             2049..2063
                     /label=BBa_B0030
     CDS             2070..2789
                     /label=GFP protein
     CDS             2070..2789
                     /label=BBa_E0040
     stem_loop       2809..2852
                     /label=stem_loop
     misc_feature    2798..2877
                     /label=BBa_B0010
     terminator      2798..2877
                     /label=BBa_B0010
     stem_loop       2893..2912
                     /label=T7 TE
     misc_feature    2919..2919
                     /label=stop
     polyA_site      2913..2926
                     /label=polya
     misc_feature    2886..2926
                     /label=BBa_B0012
     terminator      2886..2926
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gtgaatgtga
      901 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
      961 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
     1021 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     1081 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     1141 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     1201 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     1261 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     1321 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     1381 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     1441 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     1501 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     1561 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
     1621 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     1681 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     1741 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     1801 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     1861 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
     1921 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg
     1981 caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc
     2041 tactagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag
     2101 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     2161 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     2221 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     2281 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     2341 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     2401 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     2461 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     2521 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     2581 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     2641 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     2701 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     2761 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag
     2821 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     2881 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//