##gff-version 3
##sequence-region BBa_I732010 1 2926
BBa_I732010	.	ribosome_entry_site	870	881	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732010	.	conserved_region	874	877	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732010	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732010	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732010	.	CDS	2070	2789	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I732010	.	CDS	2070	2789	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I732010	.	CDS	888	2015	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I732010	.	engineered_tag	2016	2040	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I732010	.	engineered_region	888	2015	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I732010	.	tag	1977	2015	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I732010	.	CDS	888	2015	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I732010	.	ribosome_entry_site	2049	2063	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I732010	.	ribosome_entry_site	2056	2060	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I732010	.	engineered_region	2049	2063	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I732010	.	ribosome_entry_site	2049	2063	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I732010	.	terminator	2798	2877	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I732010	.	engineered_region	2798	2877	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732010	.	stem_loop	2809	2852	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732010	.	terminator	2886	2926	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I732010	.	stop_codon	2919	2919	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732010	.	polyA_site	2913	2926	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732010	.	stem_loop	2893	2912	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732010	.	engineered_region	2886	2926	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732010	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I732010	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I732010	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I732010	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I732010	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I732010	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I732010	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I732010	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I732010	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I732010	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I732010	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I732010	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I732010	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_I732010
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagattaaa
gaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatg
ttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttg
cactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatac
ccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttca
aagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaagg
tattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatg
gcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcag
accattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgc
cctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggc
atggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
ata