LOCUS       BBa_I732012             2304 bp    DNA     linear       26-Dec-2024
DEFINITION  S03608.S03518.I13507
ACCESSION   BBa_I732012
VERSION     BBa_I732012.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    661..692
                     /label=LVA
     CDS             82..660
                     /label=luxI
     misc_feature    82..699
                     /label=BBa_C0061
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             82..699
                     /label=BBa_C0061
     misc_feature    737..740
                     /label=conserved
     RBS             733..744
                     /label=BBa_B0034
     CDS             754..1370
                     /label=tetR
     misc_feature    1371..1404
                     /label=SsrA
     misc_feature    1411..1435
                     /label=Help:Barcodes
     CDS             751..1410
                     /label=BBa_C0040
     misc_feature    1448..1451
                     /label=conserved
     RBS             1444..1455
                     /label=BBa_B0034
     CDS             1462..2136
                     /label=mrfp1
     misc_feature    2143..2167
                     /label=Help:Barcodes
     CDS             1462..2142
                     /label=BBa_E1010
     stem_loop       2187..2230
                     /label=stem_loop
     misc_feature    2176..2255
                     /label=BBa_B0010
     terminator      2176..2255
                     /label=BBa_B0010
     stem_loop       2271..2290
                     /label=T7 TE
     misc_feature    2297..2297
                     /label=stop
     polyA_site      2291..2304
                     /label=polya
     misc_feature    2264..2304
                     /label=BBa_B0012
     terminator      2264..2304
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      721 tctctactag agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt
      781 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
      841 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
      901 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
      961 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     1021 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     1081 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     1141 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     1201 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     1261 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     1321 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac
     1381 gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta
     1441 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
     1501 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
     1561 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
     1621 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
     1681 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
     1741 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
     1801 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
     1861 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
     1921 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
     1981 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
     2041 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
     2101 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
     2161 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2221 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2281 cgggtgggcc tttctgcgtt tata
//