LOCUS BBa_I732071 4015 bp DNA linear 22-Dec-2024 DEFINITION Double Reporters (LacZ and GFP-AAV) ACCESSION BBa_I732071 VERSION BBa_I732071.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 3042..3042 /label=C->T CDS 19..3093 /label=lacZ misc_feature 19..21 /label=START misc_feature 3091..3093 /label=STOP CDS 19..3093 /label=BBa_I732005 misc_feature 3106..3109 /label=conserved RBS 3102..3113 /label=BBa_B0034 misc_feature 3120..3878 /label=BBa_E0044 stem_loop 3898..3941 /label=stem_loop misc_feature 3887..3966 /label=BBa_B0010 terminator 3887..3966 /label=BBa_B0010 stem_loop 3982..4001 /label=T7 TE misc_feature 4008..4008 /label=stop polyA_site 4002..4015 /label=polya misc_feature 3975..4015 /label=BBa_B0012 terminator 3975..4015 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt 61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 241 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 301 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt 361 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 421 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 481 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 541 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac 601 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 661 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 721 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 781 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 841 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 901 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 961 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 1021 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 1081 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 1141 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 1201 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 1261 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 1321 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 1381 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 1441 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 1501 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 1561 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 1621 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 1681 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 1741 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 1801 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 1861 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 1921 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 1981 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 2041 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 2101 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac 2161 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg 2221 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 2281 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 2341 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 2401 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 2461 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 2521 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 2581 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 2641 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 2701 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 2761 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 2821 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 2881 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 2941 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 3001 ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag cgccggtcgc 3061 taccattacc agttggtctg gtgtcaaaaa taatactaga gaaagaggag aaatactaga 3121 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg 3181 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa 3241 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg 3301 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc 3361 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca 3421 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta 3481 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat 3541 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa 3601 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc 3661 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc 3721 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc 3781 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaaaggcctg 3841 ctgcaaacga cgaaaactac gctgctgctg tttaataata ctagagccag gcatcaaata 3901 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3961 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //