LOCUS BBa_I732075 4042 bp DNA linear 25-Nov-2024 DEFINITION Repression-assay Part ACCESSION BBa_I732075 VERSION BBa_I732075.1 FEATURES Location/Qualifiers misc_feature 3..8 /label=BglII misc_feature 12..17 /label=BamHI misc_feature 1..19 /label=BBa_I732007 misc_feature 32..35 /label=conserved RBS 28..39 /label=BBa_B0034 misc_feature 3069..3069 /label=C->T CDS 46..3120 /label=lacZ misc_feature 46..48 /label=START misc_feature 3118..3120 /label=STOP CDS 46..3120 /label=BBa_I732005 misc_feature 3133..3136 /label=conserved RBS 3129..3140 /label=BBa_B0034 misc_feature 3147..3905 /label=BBa_E0044 stem_loop 3925..3968 /label=stem_loop misc_feature 3914..3993 /label=BBa_B0010 terminator 3914..3993 /label=BBa_B0010 stem_loop 4009..4028 /label=T7 TE misc_feature 4035..4035 /label=stop polyA_site 4029..4042 /label=polya misc_feature 4002..4042 /label=BBa_B0012 terminator 4002..4042 /label=BBa_B0012 ORIGIN 1 gaagatcttc cggatccggt actagagaaa gaggagaaat actagatgac catgattacg 61 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 121 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg 241 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc 301 gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc 361 tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg 421 ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat 481 ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac 541 agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc 601 gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg 661 atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat 721 ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt 781 cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa 841 acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt 901 tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa 961 atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa 1021 gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg 1081 aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt 1141 caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt 1201 aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc 1261 tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat 1321 cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg 1381 cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac 1441 ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg 1501 cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg 1561 cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt 1621 tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac 1681 agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc 1741 ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg 1801 tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac 1861 ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa acaccagcag 1921 cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc 1981 cgtcatagcg ataacgagct cctgcactgg atggtggcgc tggatggtaa gccgctggca 2041 agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga actgcctgaa 2101 ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt gcaaccgaac 2161 gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg tctggcggaa 2221 aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa 2281 atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt 2341 ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg cgatcagttc 2401 acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac 2461 gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag 2521 tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc gtggcagcat 2581 caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg 2641 gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg gattggcctg 2701 aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg gccgcaagaa 2761 aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac 2821 atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg 2881 aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa 2941 cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg 3001 aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg 3061 gcggaatttc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa 3121 tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg 3181 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag 3241 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa 3301 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg 3361 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt 3421 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag 3481 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag 3541 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca 3601 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg 3661 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc 3721 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca 3781 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg 3841 gcatggatga actatacaaa aggcctgctg caaacgacga aaactacgct gctgctgttt 3901 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3961 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 4021 ggtgggcctt tctgcgttta ta //