LOCUS       BBa_I732087              827 bp    DNA     linear       22-Dec-2024
DEFINITION  Tet repressor generator (TERM+)
ACCESSION   BBa_I732087
VERSION     BBa_I732087.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     CDS             64..690
                     /label=tetR
     CDS             64..690
                     /label=BBa_I732080
     stem_loop       710..753
                     /label=stem_loop
     misc_feature    699..778
                     /label=BBa_B0010
     terminator      699..778
                     /label=BBa_B0010
     stem_loop       794..813
                     /label=T7 TE
     misc_feature    820..820
                     /label=stop
     polyA_site      814..827
                     /label=polya
     misc_feature    787..827
                     /label=BBa_B0012
     terminator      787..827
                     /label=BBa_B0012
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
      121 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
      181 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
      241 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
      301 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
      361 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
      421 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
      481 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
      541 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
      601 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
      661 caacttaaat gtgaaagtgg gtcctaataa tactagagcc aggcatcaaa taaaacgaaa
      721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      781 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//