LOCUS       BBa_I732091             1174 bp    DNA     linear       12-May-2024
DEFINITION  Double Repoters (LacZ-alpha and GFP-AAV)
ACCESSION   BBa_I732091
VERSION     BBa_I732091.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..252
                     /label=lacZ
     misc_feature    19..21
                     /label=START
     misc_feature    247..252
                     /label=STOP
     CDS             19..252
                     /label=BBa_I732006
     misc_feature    265..268
                     /label=conserved
     RBS             261..272
                     /label=BBa_B0034
     misc_feature    279..1037
                     /label=BBa_E0044
     stem_loop       1057..1100
                     /label=stem_loop
     misc_feature    1046..1125
                     /label=BBa_B0010
     terminator      1046..1125
                     /label=BBa_B0010
     stem_loop       1141..1160
                     /label=T7 TE
     misc_feature    1167..1167
                     /label=stop
     polyA_site      1161..1174
                     /label=polya
     misc_feature    1134..1174
                     /label=BBa_B0012
     terminator      1134..1174
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt
       61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
      241 ctggagtaat aatactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt
      301 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      361 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
      421 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
      481 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
      541 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      601 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
      661 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
      721 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
      781 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
      841 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
      901 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
      961 ggattacaca tggcatggat gaactataca aaaggcctgc tgcaaacgac gaaaactacg
     1021 ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1081 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1141 ggctcacctt cgggtgggcc tttctgcgtt tata
//