LOCUS       BBa_I732092             1121 bp    DNA     linear       22-Dec-2024
DEFINITION  Double Repoters (LacZ-alpha and RFP)
ACCESSION   BBa_I732092
VERSION     BBa_I732092.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..252
                     /label=lacZ
     misc_feature    19..21
                     /label=START
     misc_feature    247..252
                     /label=STOP
     CDS             19..252
                     /label=BBa_I732006
     misc_feature    1..252
                     /label=BBa_I732018
     misc_feature    265..268
                     /label=conserved
     RBS             261..272
                     /label=BBa_B0034
     CDS             279..953
                     /label=mrfp1
     misc_feature    960..984
                     /label=Help:Barcodes
     CDS             279..984
                     /label=BBa_E1010
     stem_loop       1004..1047
                     /label=stem_loop
     misc_feature    993..1072
                     /label=BBa_B0010
     terminator      993..1072
                     /label=BBa_B0010
     stem_loop       1088..1107
                     /label=T7 TE
     misc_feature    1114..1114
                     /label=stop
     polyA_site      1108..1121
                     /label=polya
     misc_feature    1081..1121
                     /label=BBa_B0012
     terminator      1081..1121
                     /label=BBa_B0012
     terminator      993..1121
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt
       61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
      241 ctggagtaat aatactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta
      301 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
      361 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
      421 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
      481 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
      541 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
      601 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
      661 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
      721 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
      781 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
      841 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
      901 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
      961 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     1021 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1081 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//