LOCUS       BBa_I732094             1201 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter Activity Reporter (LacZ-alpha and GFP-AAV)
ACCESSION   BBa_I732094
VERSION     BBa_I732094.1  
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label=BglII
     misc_feature    12..17
                     /label=BamHI
     misc_feature    1..19
                     /label=BBa_I732007
     misc_feature    32..35
                     /label=conserved
     RBS             28..39
                     /label=BBa_B0034
     CDS             46..279
                     /label=lacZ
     misc_feature    46..48
                     /label=START
     misc_feature    274..279
                     /label=STOP
     CDS             46..279
                     /label=BBa_I732006
     misc_feature    292..295
                     /label=conserved
     RBS             288..299
                     /label=BBa_B0034
     misc_feature    306..1064
                     /label=BBa_E0044
     stem_loop       1084..1127
                     /label=stem_loop
     misc_feature    1073..1152
                     /label=BBa_B0010
     terminator      1073..1152
                     /label=BBa_B0010
     stem_loop       1168..1187
                     /label=T7 TE
     misc_feature    1194..1194
                     /label=stop
     polyA_site      1188..1201
                     /label=polya
     misc_feature    1161..1201
                     /label=BBa_B0012
     terminator      1161..1201
                     /label=BBa_B0012
ORIGIN
        1 gaagatcttc cggatccggt actagagaaa gaggagaaat actagatgac catgattacg
       61 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      121 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
      241 gcaccagaag cggtgccgga aagctggctg gagtaataat actagagaaa gaggagaaat
      301 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      361 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      421 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      481 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      541 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      601 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      661 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      721 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      781 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      841 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      901 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      961 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaaa
     1021 ggcctgctgc aaacgacgaa aactacgctg ctgctgttta ataatactag agccaggcat
     1081 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1141 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1201 a
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