LOCUS       BBa_I732095             1148 bp    DNA     linear       08-May-2024
DEFINITION  Promoter Activity Reporter (LacZ-alpha and RFP)
ACCESSION   BBa_I732095
VERSION     BBa_I732095.1  
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label=BglII
     misc_feature    12..17
                     /label=BamHI
     misc_feature    1..19
                     /label=BBa_I732007
     misc_feature    32..35
                     /label=conserved
     RBS             28..39
                     /label=BBa_B0034
     CDS             46..279
                     /label=lacZ
     misc_feature    46..48
                     /label=START
     misc_feature    274..279
                     /label=STOP
     CDS             46..279
                     /label=BBa_I732006
     misc_feature    1..279
                     /label=BBa_I732074
     misc_feature    292..295
                     /label=conserved
     RBS             288..299
                     /label=BBa_B0034
     CDS             306..980
                     /label=mrfp1
     misc_feature    987..1011
                     /label=Help:Barcodes
     CDS             306..986
                     /label=BBa_E1010
     stem_loop       1031..1074
                     /label=stem_loop
     misc_feature    1020..1099
                     /label=BBa_B0010
     terminator      1020..1099
                     /label=BBa_B0010
     stem_loop       1115..1134
                     /label=T7 TE
     misc_feature    1141..1141
                     /label=stop
     polyA_site      1135..1148
                     /label=polya
     misc_feature    1108..1148
                     /label=BBa_B0012
     terminator      1108..1148
                     /label=BBa_B0012
     misc_feature    288..1148
                     /label=BBa_I13507
ORIGIN
        1 gaagatcttc cggatccggt actagagaaa gaggagaaat actagatgac catgattacg
       61 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      121 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
      241 gcaccagaag cggtgccgga aagctggctg gagtaataat actagagaaa gaggagaaat
      301 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
      361 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
      421 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
      481 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
      541 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
      601 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
      661 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
      721 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
      781 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
      841 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
      901 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
      961 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     1021 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1081 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1141 cgtttata
//