LOCUS       BBa_I732096             1162 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter Activity Reporter (LacZ-alpha and GFP)
ACCESSION   BBa_I732096
VERSION     BBa_I732096.1  
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label=BglII
     misc_feature    12..17
                     /label=BamHI
     misc_feature    1..19
                     /label=BBa_I732007
     misc_feature    32..35
                     /label=conserved
     RBS             28..39
                     /label=BBa_B0034
     CDS             46..279
                     /label=lacZ
     misc_feature    46..48
                     /label=START
     misc_feature    274..279
                     /label=STOP
     CDS             46..279
                     /label=BBa_I732006
     misc_feature    292..295
                     /label=conserved
     RBS             288..299
                     /label=BBa_B0034
     CDS             306..1025
                     /label=GFP protein
     CDS             306..1025
                     /label=BBa_E0040
     stem_loop       1045..1088
                     /label=stem_loop
     misc_feature    1034..1113
                     /label=BBa_B0010
     terminator      1034..1113
                     /label=BBa_B0010
     stem_loop       1129..1148
                     /label=T7 TE
     misc_feature    1155..1155
                     /label=stop
     polyA_site      1149..1162
                     /label=polya
     misc_feature    1122..1162
                     /label=BBa_B0012
     terminator      1122..1162
                     /label=BBa_B0012
ORIGIN
        1 gaagatcttc cggatccggt actagagaaa gaggagaaat actagatgac catgattacg
       61 gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      121 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      181 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
      241 gcaccagaag cggtgccgga aagctggctg gagtaataat actagagaaa gaggagaaat
      301 actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      361 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      421 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      481 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga
      541 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
      601 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      661 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      721 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      781 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      841 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      901 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      961 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     1021 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1081 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1141 ggtgggcctt tctgcgttta ta
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