LOCUS       BBa_I732097              888 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter Activity Reporter (RFP)
ACCESSION   BBa_I732097
VERSION     BBa_I732097.1  
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label=BglII
     misc_feature    12..17
                     /label=BamHI
     misc_feature    1..19
                     /label=BBa_I732007
     misc_feature    32..35
                     /label=conserved
     RBS             28..39
                     /label=BBa_B0034
     CDS             46..720
                     /label=mrfp1
     misc_feature    727..751
                     /label=Help:Barcodes
     CDS             46..751
                     /label=BBa_E1010
     stem_loop       771..814
                     /label=stem_loop
     misc_feature    760..839
                     /label=BBa_B0010
     terminator      760..839
                     /label=BBa_B0010
     stem_loop       855..874
                     /label=T7 TE
     misc_feature    881..881
                     /label=stop
     polyA_site      875..888
                     /label=polya
     misc_feature    848..888
                     /label=BBa_B0012
     terminator      848..888
                     /label=BBa_B0012
     terminator      760..888
                     /label=BBa_B0015
ORIGIN
        1 gaagatcttc cggatccggt actagagaaa gaggagaaat actagatggc ttcctccgaa
       61 gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac
      121 gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa
      181 ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc
      241 cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg
      301 tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt
      361 accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt
      421 ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct
      481 tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg
      541 aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa
      601 ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac
      661 gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct
      721 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
      781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//