LOCUS       BBa_I732716             1267 bp    DNA     linear       27-Dec-2024
DEFINITION  P_NOT_D001O47 + Double Reporters
ACCESSION   BBa_I732716
VERSION     BBa_I732716.1  
FEATURES             Location/Qualifiers
     promoter        1..124
                     /label=BBa_I732278
     misc_feature    137..140
                     /label=conserved
     RBS             133..144
                     /label=BBa_B0034
     CDS             151..384
                     /label=lacZ
     misc_feature    151..153
                     /label=START
     misc_feature    379..384
                     /label=STOP
     CDS             151..384
                     /label=BBa_I732006
     misc_feature    397..400
                     /label=conserved
     RBS             393..404
                     /label=BBa_B0034
     CDS             411..1130
                     /label=GFP protein
     CDS             411..1130
                     /label=BBa_E0040
     stem_loop       1150..1193
                     /label=stem_loop
     misc_feature    1139..1218
                     /label=BBa_B0010
     terminator      1139..1218
                     /label=BBa_B0010
     stem_loop       1234..1253
                     /label=T7 TE
     misc_feature    1260..1260
                     /label=stop
     polyA_site      1254..1267
                     /label=polya
     misc_feature    1227..1267
                     /label=BBa_B0012
     terminator      1227..1267
                     /label=BBa_B0012
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgaactaca gtcgtcggat
      121 ccggtactag agaaagagga gaaatactag atgaccatga ttacggattc actggccgtc
      181 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      241 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      301 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
      361 ccggaaagct ggctggagta ataatactag agaaagagga gaaatactag atgcgtaaag
      421 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      481 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      541 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      601 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      661 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      721 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      781 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      841 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      901 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      961 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1021 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1081 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
     1141 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1201 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1261 gtttata
//