LOCUS       BBa_I732727             1316 bp    DNA     linear       22-Nov-2024
DEFINITION  P_U085O11+D062NUL + Double Reporters
ACCESSION   BBa_I732727
VERSION     BBa_I732727.1  
FEATURES             Location/Qualifiers
     promoter        69..105
                     /label=consensus elements
     misc_feature    1..20
                     /label=O1
     misc_feature    105..105
                     /label=+1
     promoter        1..173
                     /label=BBa_I732402
     misc_feature    186..189
                     /label=conserved
     RBS             182..193
                     /label=BBa_B0034
     CDS             200..433
                     /label=lacZ
     misc_feature    200..202
                     /label=START
     misc_feature    428..433
                     /label=STOP
     CDS             200..433
                     /label=BBa_I732006
     misc_feature    446..449
                     /label=conserved
     RBS             442..453
                     /label=BBa_B0034
     CDS             460..1179
                     /label=GFP protein
     CDS             460..1179
                     /label=BBa_E0040
     stem_loop       1199..1242
                     /label=stem_loop
     misc_feature    1188..1267
                     /label=BBa_B0010
     terminator      1188..1267
                     /label=BBa_B0010
     stem_loop       1283..1302
                     /label=T7 TE
     misc_feature    1309..1309
                     /label=stop
     polyA_site      1303..1316
                     /label=polya
     misc_feature    1276..1316
                     /label=BBa_B0012
     terminator      1276..1316
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc gctcacaatt aattcgagac cggaactcga ttgtatctgt agtgctttag
       61 tagtggagtt tacactttat gcttccggct cgtataatgt gtggaacatc ccctttatct
      121 atcgcctgtg cccactacat caagccaaat taaacaggat taacaggatc cggtactaga
      181 gaaagaggag aaatactaga tgaccatgat tacggattca ctggccgtcg ttttacaacg
      241 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      301 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      361 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
      421 gctggagtaa taatactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt
      481 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      541 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      601 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
      661 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
      721 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      781 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      841 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      901 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
      961 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1021 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     1081 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     1141 gggattacac atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat
     1201 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1261 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//