LOCUS BBa_I732728 1304 bp DNA linear 22-Dec-2024 DEFINITION P_U073O11+D062NUL + Double Reporters ACCESSION BBa_I732728 VERSION BBa_I732728.1 FEATURES Location/Qualifiers promoter 57..93 /label=consensus elements misc_feature 1..20 /label=O1 misc_feature 93..93 /label=+1 promoter 1..161 /label=BBa_I732403 misc_feature 174..177 /label=conserved RBS 170..181 /label=BBa_B0034 CDS 188..421 /label=lacZ misc_feature 188..190 /label=START misc_feature 416..421 /label=STOP CDS 188..421 /label=BBa_I732006 misc_feature 434..437 /label=conserved RBS 430..441 /label=BBa_B0034 CDS 448..1167 /label=GFP protein CDS 448..1167 /label=BBa_E0040 stem_loop 1187..1230 /label=stem_loop misc_feature 1176..1255 /label=BBa_B0010 terminator 1176..1255 /label=BBa_B0010 stem_loop 1271..1290 /label=T7 TE misc_feature 1297..1297 /label=stop polyA_site 1291..1304 /label=polya misc_feature 1264..1304 /label=BBa_B0012 terminator 1264..1304 /label=BBa_B0012 ORIGIN 1 aattgtgagc gctcacaatt gaactcgatt gtatctgtag tgctttagta gtggagttta 61 cactttatgc ttccggctcg tataatgtgt ggaacatccc ctttatctat cgcctgtgcc 121 cactacatca agccaaatta aacaggatta acaggatccg gtactagaga aagaggagaa 181 atactagatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga 241 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 301 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 361 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtaata 421 atactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt 481 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga 541 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga 601 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt 661 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt 721 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa 781 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa 841 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac 901 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt 961 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc 1021 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc 1081 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat 1141 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc 1201 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1261 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //