BBa_I732729
type: ComponentDefinition
persistent identity: BBa I732729
display id: BBa_I732729
version: 1
was generated by: iGEM to SBOL conversion
description: P_U061O11+D062NUL + Double Reporters
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:59Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: N/A
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_B0034
component: BBa_I732006
component: BBa_I732404
sequence annotation: BBa_I732404
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732729_sequence/1

BBa_B0012
type: Component
persistent identity: component1948064
display id: component1948064
version: 1
top level: BBa_I732729
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1948062
display id: component1948062
version: 1
top level: BBa_I732729
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1948061
display id: component1948061
version: 1
top level: BBa_I732729
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1948059
display id: component1948059
version: 1
top level: BBa_I732729
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1948057
display id: component1948057
version: 1
top level: BBa_I732729
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1948053
display id: component1948053
version: 1
top level: BBa_I732729
definition: BBa_B0034
access: public

BBa_I732404
type: Component
persistent identity: component1948051
display id: component1948051
version: 1
top level: BBa_I732729
definition: U061O11+D0
access: public

BBa_B0012
type: Component
persistent identity: component1948047
display id: component1948047
version: 1
top level: BBa_I732729
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1948045
display id: component1948045
version: 1
top level: BBa_I732729
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1948042
display id: component1948042
version: 1
top level: BBa_I732729
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1948039
display id: component1948039
version: 1
top level: BBa_I732729
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1948036
display id: component1948036
version: 1
top level: BBa_I732729
definition: BBa_B0034
access: public

BBa_I732404
type: SequenceAnnotation
persistent identity: annotation1948051
display id: annotation1948051
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948051/range1948051/1
component: BBa_I732404

https://synbiohub.org/public/igem/BBa_I732729/annotation1948051/range1948051/1
type: Range
persistent identity: range1948051
display id: range1948051
version: 1
top level: BBa_I732729
direction: direction/reverse
start: 1
end: 149
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948036
display id: annotation1948036
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948036/range1948036/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732729/annotation1948036/range1948036/1
type: Range
persistent identity: range1948036
display id: range1948036
version: 1
top level: BBa_I732729
direction: direction/reverse
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948039
display id: annotation1948039
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948039/range1948039/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732729/annotation1948039/range1948039/1
type: Range
persistent identity: range1948039
display id: range1948039
version: 1
top level: BBa_I732729
direction: direction/reverse
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948053
display id: annotation1948053
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948053/range1948053/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732729/annotation1948053/range1948053/1
type: Range
persistent identity: range1948053
display id: range1948053
version: 1
top level: BBa_I732729
direction: direction/reverse
start: 158
end: 169
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948057
display id: annotation1948057
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948057/range1948057/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732729/annotation1948057/range1948057/1
type: Range
persistent identity: range1948057
display id: range1948057
version: 1
top level: BBa_I732729
direction: direction/reverse
start: 176
end: 409
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948042
display id: annotation1948042
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948042/range1948042/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732729/annotation1948042/range1948042/1
type: Range
persistent identity: range1948042
display id: range1948042
version: 1
top level: BBa_I732729
direction: direction/reverse
start: 288
end: 299
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948059
display id: annotation1948059
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948059/range1948059/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732729/annotation1948059/range1948059/1
type: Range
persistent identity: range1948059
display id: range1948059
version: 1
top level: BBa_I732729
direction: direction/reverse
start: 418
end: 429
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1948061
display id: annotation1948061
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948061/range1948061/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I732729/annotation1948061/range1948061/1
type: Range
persistent identity: range1948061
display id: range1948061
version: 1
top level: BBa_I732729
direction: direction/reverse
start: 436
end: 1155
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948045
display id: annotation1948045
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948045/range1948045/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732729/annotation1948045/range1948045/1
type: Range
persistent identity: range1948045
display id: range1948045
version: 1
top level: BBa_I732729
direction: direction/forward
start: 1073
end: 1152
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948047
display id: annotation1948047
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948047/range1948047/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732729/annotation1948047/range1948047/1
type: Range
persistent identity: range1948047
display id: range1948047
version: 1
top level: BBa_I732729
direction: direction/forward
start: 1161
end: 1201
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948062
display id: annotation1948062
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948062/range1948062/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732729/annotation1948062/range1948062/1
type: Range
persistent identity: range1948062
display id: range1948062
version: 1
top level: BBa_I732729
direction: direction/forward
start: 1164
end: 1243
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948064
display id: annotation1948064
version: 1
top level: BBa_I732729
location: https://synbiohub.org/public/igem/BBa_I732729/annotation1948064/range1948064/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732729/annotation1948064/range1948064/1
type: Range
persistent identity: range1948064
display id: range1948064
version: 1
top level: BBa_I732729
direction: direction/forward
start: 1252
end: 1292
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ alpha fragment
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 234
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 229
end: 234
orientation: inline

U061O11+D0
type: ComponentDefinition
persistent identity: BBa I732404
display id: BBa_I732404
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Family Member (U061O11+D062NUL)
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
mutable provenance: Might be later edited.
owned by: james
owned by: myers
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732401.
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Might be later edited.
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BglII
sequence annotation: BamHI
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: lacZ
sequence annotation: BBa_I732006
sequence annotation: START
sequence annotation: STOP
sequence annotation: BBa_E0044
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I732404_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation1947684
display id: annotation1947684
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I732404/annotation1947684/range1947684/1
type: Range
persistent identity: range1947684
display id: range1947684
version: 1
top level: U061O11+D0
direction: direction/forward
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1947685
display id: annotation1947685
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I732404/annotation1947685/range1947685/1
type: Range
persistent identity: range1947685
display id: range1947685
version: 1
top level: U061O11+D0
direction: direction/forward
start: 12
end: 17
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947686
display id: annotation1947686
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732404/annotation1947686/range1947686/1
type: Range
persistent identity: range1947686
display id: range1947686
version: 1
top level: U061O11+D0
direction: direction/reverse
start: 28
end: 39
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947687
display id: annotation1947687
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_I732404/annotation1947687/range1947687/1
type: Range
persistent identity: range1947687
display id: range1947687
version: 1
top level: U061O11+D0
direction: direction/forward
start: 32
end: 35
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1947689
display id: annotation1947689
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732404/annotation1947689/range1947689/1
type: Range
persistent identity: range1947689
display id: range1947689
version: 1
top level: U061O11+D0
direction: direction/forward
start: 46
end: 279
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1947688
display id: annotation1947688
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732404/annotation1947688/range1947688/1
type: Range
persistent identity: range1947688
display id: range1947688
version: 1
top level: U061O11+D0
direction: direction/reverse
start: 46
end: 279
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1947690
display id: annotation1947690
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I732404/annotation1947690/range1947690/1
type: Range
persistent identity: range1947690
display id: range1947690
version: 1
top level: U061O11+D0
direction: direction/forward
start: 46
end: 48
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1947691
display id: annotation1947691
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732404/annotation1947691/range1947691/1
type: Range
persistent identity: range1947691
display id: range1947691
version: 1
top level: U061O11+D0
direction: direction/forward
start: 274
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947692
display id: annotation1947692
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732404/annotation1947692/range1947692/1
type: Range
persistent identity: range1947692
display id: range1947692
version: 1
top level: U061O11+D0
direction: direction/reverse
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1947693
display id: annotation1947693
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_I732404/annotation1947693/range1947693/1
type: Range
persistent identity: range1947693
display id: range1947693
version: 1
top level: U061O11+D0
direction: direction/forward
start: 292
end: 295
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1947694
display id: annotation1947694
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732404/annotation1947694/range1947694/1
type: Range
persistent identity: range1947694
display id: range1947694
version: 1
top level: U061O11+D0
direction: direction/reverse
start: 306
end: 1064
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1947695
display id: annotation1947695
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732404/annotation1947695/range1947695/1
type: Range
persistent identity: range1947695
display id: range1947695
version: 1
top level: U061O11+D0
direction: direction/forward
start: 1073
end: 1152
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1947696
display id: annotation1947696
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I732404/annotation1947696/range1947696/1
type: Range
persistent identity: range1947696
display id: range1947696
version: 1
top level: U061O11+D0
direction: direction/forward
start: 1084
end: 1127
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1947697
display id: annotation1947697
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732404/annotation1947697/range1947697/1
type: Range
persistent identity: range1947697
display id: range1947697
version: 1
top level: U061O11+D0
direction: direction/forward
start: 1161
end: 1201
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1947698
display id: annotation1947698
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I732404/annotation1947698/range1947698/1
type: Range
persistent identity: range1947698
display id: range1947698
version: 1
top level: U061O11+D0
direction: direction/forward
start: 1168
end: 1187
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1947699
display id: annotation1947699
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_I732404/annotation1947699/range1947699/1
type: Range
persistent identity: range1947699
display id: range1947699
version: 1
top level: U061O11+D0
direction: direction/forward
start: 1188
end: 1201
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1947700
display id: annotation1947700
version: 1
top level: U061O11+D0
location: https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732404/annotation1947700/range1947700/1
type: Range
persistent identity: range1947700
display id: range1947700
version: 1
top level: U061O11+D0
direction: direction/forward
start: 1194
end: 1194
orientation: inline

https://synbiohub.org/public/igem/BBa_I732729_sequence/1
type: Sequence
persistent identity: BBa I732729 sequence
display id: BBa_I732729_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcgctcacaattatctgtagtgctttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaattaaacaggattaacaggatccggtactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732006_sequence/1
type: Sequence
persistent identity: BBa I732006 sequence
display id: BBa_I732006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732404_sequence/1
type: Sequence
persistent identity: BBa I732404 sequence
display id: BBa_I732404_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcgctcacaattatctgtagtgctttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaattaaacaggattaacaggatccgg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z