BBa_I732730
type: ComponentDefinition
persistent identity: BBa I732730
display id: BBa_I732730
version: 1
was generated by: iGEM to SBOL conversion
description: P_U049O11+D062NUL + Double Reporters
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:59Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: N/A
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I732093
component: BBa_I732405
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_I732006
sequence annotation: BBa_I732405
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_I732093
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732730_sequence/1

BBa_I732093
type: Component
persistent identity: component2266091
display id: component2266091
version: 1
top level: BBa_I732730
definition: BBa_I732093
access: public

BBa_I732405
type: Component
persistent identity: component2266074
display id: component2266074
version: 1
top level: BBa_I732730
definition: U049O11+D0
access: public

BBa_B0012
type: Component
persistent identity: component2266070
display id: component2266070
version: 1
top level: BBa_I732730
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2266068
display id: component2266068
version: 1
top level: BBa_I732730
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component2266063
display id: component2266063
version: 1
top level: BBa_I732730
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component2266059
display id: component2266059
version: 1
top level: BBa_I732730
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component2266057
display id: component2266057
version: 1
top level: BBa_I732730
definition: BBa_B0034
access: public

BBa_I732405
type: SequenceAnnotation
persistent identity: annotation2266074
display id: annotation2266074
version: 1
top level: BBa_I732730
location: https://synbiohub.org/public/igem/BBa_I732730/annotation2266074/range2266074/1
component: BBa_I732405

https://synbiohub.org/public/igem/BBa_I732730/annotation2266074/range2266074/1
type: Range
persistent identity: range2266074
display id: range2266074
version: 1
top level: BBa_I732730
direction: direction/reverse
start: 1
end: 137
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2266057
display id: annotation2266057
version: 1
top level: BBa_I732730
location: https://synbiohub.org/public/igem/BBa_I732730/annotation2266057/range2266057/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732730/annotation2266057/range2266057/1
type: Range
persistent identity: range2266057
display id: range2266057
version: 1
top level: BBa_I732730
direction: direction/reverse
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation2266059
display id: annotation2266059
version: 1
top level: BBa_I732730
location: https://synbiohub.org/public/igem/BBa_I732730/annotation2266059/range2266059/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732730/annotation2266059/range2266059/1
type: Range
persistent identity: range2266059
display id: range2266059
version: 1
top level: BBa_I732730
direction: direction/reverse
start: 46
end: 279
orientation: inline

BBa_I732093
type: SequenceAnnotation
persistent identity: annotation2266091
display id: annotation2266091
version: 1
top level: BBa_I732730
location: https://synbiohub.org/public/igem/BBa_I732730/annotation2266091/range2266091/1
component: BBa_I732093

https://synbiohub.org/public/igem/BBa_I732730/annotation2266091/range2266091/1
type: Range
persistent identity: range2266091
display id: range2266091
version: 1
top level: BBa_I732730
direction: direction/reverse
start: 146
end: 1280
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2266063
display id: annotation2266063
version: 1
top level: BBa_I732730
location: https://synbiohub.org/public/igem/BBa_I732730/annotation2266063/range2266063/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732730/annotation2266063/range2266063/1
type: Range
persistent identity: range2266063
display id: range2266063
version: 1
top level: BBa_I732730
direction: direction/reverse
start: 288
end: 299
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2266068
display id: annotation2266068
version: 1
top level: BBa_I732730
location: https://synbiohub.org/public/igem/BBa_I732730/annotation2266068/range2266068/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732730/annotation2266068/range2266068/1
type: Range
persistent identity: range2266068
display id: range2266068
version: 1
top level: BBa_I732730
direction: direction/forward
start: 1020
end: 1099
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2266070
display id: annotation2266070
version: 1
top level: BBa_I732730
location: https://synbiohub.org/public/igem/BBa_I732730/annotation2266070/range2266070/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732730/annotation2266070/range2266070/1
type: Range
persistent identity: range2266070
display id: range2266070
version: 1
top level: BBa_I732730
direction: direction/forward
start: 1108
end: 1148
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ alpha fragment
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 234
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 229
end: 234
orientation: inline

U049O11+D0
type: ComponentDefinition
persistent identity: BBa I732405
display id: BBa_I732405
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Family Member (U049O11+D062NUL)
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
mutable provenance: Might be later edited.
owned by: james
owned by: myers
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732402.
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Might be later edited.
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_I732007
sequence annotation: BglII
sequence annotation: BamHI
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: lacZ
sequence annotation: START
sequence annotation: BBa_I732006
sequence annotation: STOP
sequence annotation: BBa_E1010
sequence annotation: mrfp1
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I732405_sequence/1

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944406
display id: annotation1944406
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732405/annotation1944406/range1944406/1
type: Range
persistent identity: range1944406
display id: range1944406
version: 1
top level: U049O11+D0
direction: direction/reverse
start: 1
end: 19
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944404
display id: annotation1944404
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I732405/annotation1944404/range1944404/1
type: Range
persistent identity: range1944404
display id: range1944404
version: 1
top level: U049O11+D0
direction: direction/forward
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944405
display id: annotation1944405
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I732405/annotation1944405/range1944405/1
type: Range
persistent identity: range1944405
display id: range1944405
version: 1
top level: U049O11+D0
direction: direction/forward
start: 12
end: 17
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944408
display id: annotation1944408
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732405/annotation1944408/range1944408/1
type: Range
persistent identity: range1944408
display id: range1944408
version: 1
top level: U049O11+D0
direction: direction/reverse
start: 28
end: 39
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944407
display id: annotation1944407
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_I732405/annotation1944407/range1944407/1
type: Range
persistent identity: range1944407
display id: range1944407
version: 1
top level: U049O11+D0
direction: direction/forward
start: 32
end: 35
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944410
display id: annotation1944410
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732405/annotation1944410/range1944410/1
type: Range
persistent identity: range1944410
display id: range1944410
version: 1
top level: U049O11+D0
direction: direction/forward
start: 46
end: 279
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944409
display id: annotation1944409
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I732405/annotation1944409/range1944409/1
type: Range
persistent identity: range1944409
display id: range1944409
version: 1
top level: U049O11+D0
direction: direction/forward
start: 46
end: 48
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944412
display id: annotation1944412
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732405/annotation1944412/range1944412/1
type: Range
persistent identity: range1944412
display id: range1944412
version: 1
top level: U049O11+D0
direction: direction/reverse
start: 46
end: 279
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944411
display id: annotation1944411
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732405/annotation1944411/range1944411/1
type: Range
persistent identity: range1944411
display id: range1944411
version: 1
top level: U049O11+D0
direction: direction/forward
start: 274
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944414
display id: annotation1944414
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732405/annotation1944414/range1944414/1
type: Range
persistent identity: range1944414
display id: range1944414
version: 1
top level: U049O11+D0
direction: direction/reverse
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944413
display id: annotation1944413
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_I732405/annotation1944413/range1944413/1
type: Range
persistent identity: range1944413
display id: range1944413
version: 1
top level: U049O11+D0
direction: direction/forward
start: 292
end: 295
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944416
display id: annotation1944416
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732405/annotation1944416/range1944416/1
type: Range
persistent identity: range1944416
display id: range1944416
version: 1
top level: U049O11+D0
direction: direction/reverse
start: 306
end: 986
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944415
display id: annotation1944415
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732405/annotation1944415/range1944415/1
type: Range
persistent identity: range1944415
display id: range1944415
version: 1
top level: U049O11+D0
direction: direction/forward
start: 306
end: 980
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944417
display id: annotation1944417
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732405/annotation1944417/range1944417/1
type: Range
persistent identity: range1944417
display id: range1944417
version: 1
top level: U049O11+D0
direction: direction/reverse
start: 987
end: 1011
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944418
display id: annotation1944418
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732405/annotation1944418/range1944418/1
type: Range
persistent identity: range1944418
display id: range1944418
version: 1
top level: U049O11+D0
direction: direction/forward
start: 1020
end: 1099
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944419
display id: annotation1944419
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I732405/annotation1944419/range1944419/1
type: Range
persistent identity: range1944419
display id: range1944419
version: 1
top level: U049O11+D0
direction: direction/forward
start: 1031
end: 1074
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944420
display id: annotation1944420
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732405/annotation1944420/range1944420/1
type: Range
persistent identity: range1944420
display id: range1944420
version: 1
top level: U049O11+D0
direction: direction/forward
start: 1108
end: 1148
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944421
display id: annotation1944421
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I732405/annotation1944421/range1944421/1
type: Range
persistent identity: range1944421
display id: range1944421
version: 1
top level: U049O11+D0
direction: direction/forward
start: 1115
end: 1134
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944422
display id: annotation1944422
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_I732405/annotation1944422/range1944422/1
type: Range
persistent identity: range1944422
display id: range1944422
version: 1
top level: U049O11+D0
direction: direction/forward
start: 1135
end: 1148
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944423
display id: annotation1944423
version: 1
top level: U049O11+D0
location: https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732405/annotation1944423/range1944423/1
type: Range
persistent identity: range1944423
display id: range1944423
version: 1
top level: U049O11+D0
direction: direction/forward
start: 1141
end: 1141
orientation: inline

BBa_I732093
type: ComponentDefinition
persistent identity: BBa I732093
display id: BBa_I732093
version: 1
was generated by: iGEM to SBOL conversion
description: Double Repoters (LacZ-alpha and GFP)
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: The activity of upstream promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_I732006
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732093_sequence/1

BBa_B0034
type: Component
persistent identity: component1947026
display id: component1947026
version: 1
top level: BBa_I732093
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1947024
display id: component1947024
version: 1
top level: BBa_I732093
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1947020
display id: component1947020
version: 1
top level: BBa_I732093
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1947031
display id: component1947031
version: 1
top level: BBa_I732093
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1947029
display id: component1947029
version: 1
top level: BBa_I732093
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1947028
display id: component1947028
version: 1
top level: BBa_I732093
definition: GFP
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947020
display id: annotation1947020
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947020/range1947020/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732093/annotation1947020/range1947020/1
type: Range
persistent identity: range1947020
display id: range1947020
version: 1
top level: BBa_I732093
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1947024
display id: annotation1947024
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947024/range1947024/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732093/annotation1947024/range1947024/1
type: Range
persistent identity: range1947024
display id: range1947024
version: 1
top level: BBa_I732093
direction: direction/reverse
start: 19
end: 252
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947026
display id: annotation1947026
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947026/range1947026/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732093/annotation1947026/range1947026/1
type: Range
persistent identity: range1947026
display id: range1947026
version: 1
top level: BBa_I732093
direction: direction/reverse
start: 261
end: 272
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1947028
display id: annotation1947028
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947028/range1947028/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I732093/annotation1947028/range1947028/1
type: Range
persistent identity: range1947028
display id: range1947028
version: 1
top level: BBa_I732093
direction: direction/reverse
start: 279
end: 998
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1947029
display id: annotation1947029
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947029/range1947029/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732093/annotation1947029/range1947029/1
type: Range
persistent identity: range1947029
display id: range1947029
version: 1
top level: BBa_I732093
direction: direction/forward
start: 1007
end: 1086
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1947031
display id: annotation1947031
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947031/range1947031/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732093/annotation1947031/range1947031/1
type: Range
persistent identity: range1947031
display id: range1947031
version: 1
top level: BBa_I732093
direction: direction/forward
start: 1095
end: 1135
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_I732730_sequence/1
type: Sequence
persistent identity: BBa I732730 sequence
display id: BBa_I732730_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcgctcacaattttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaattaaacaggattaacaggatccggtactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732006_sequence/1
type: Sequence
persistent identity: BBa I732006 sequence
display id: BBa_I732006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732405_sequence/1
type: Sequence
persistent identity: BBa I732405 sequence
display id: BBa_I732405_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcgctcacaattttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaattaaacaggattaacaggatccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732093_sequence/1
type: Sequence
persistent identity: BBa I732093 sequence
display id: BBa_I732093_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z