BBa_I732731
type: ComponentDefinition
persistent identity: BBa I732731
display id: BBa_I732731
version: 1
was generated by: iGEM to SBOL conversion
description: P_U037O11+D062NUL + Double Reporters
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:59Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: N/A
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E0040
component: BBa_I732006
component: BBa_I732406
component: BBa_B0012
sequence annotation: BBa_I732406
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732731_sequence/1

BBa_B0034
type: Component
persistent identity: component1948108
display id: component1948108
version: 1
top level: BBa_I732731
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1948118
display id: component1948118
version: 1
top level: BBa_I732731
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1948117
display id: component1948117
version: 1
top level: BBa_I732731
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1948114
display id: component1948114
version: 1
top level: BBa_I732731
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1948110
display id: component1948110
version: 1
top level: BBa_I732731
definition: lacZ-alpha
access: public

BBa_I732006
type: Component
persistent identity: component1948130
display id: component1948130
version: 1
top level: BBa_I732731
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1948126
display id: component1948126
version: 1
top level: BBa_I732731
definition: BBa_B0034
access: public

BBa_I732406
type: Component
persistent identity: component1948124
display id: component1948124
version: 1
top level: BBa_I732731
definition: U037O11+D0
access: public

BBa_B0012
type: Component
persistent identity: component1948120
display id: component1948120
version: 1
top level: BBa_I732731
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component1948137
display id: component1948137
version: 1
top level: BBa_I732731
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1948135
display id: component1948135
version: 1
top level: BBa_I732731
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1948134
display id: component1948134
version: 1
top level: BBa_I732731
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1948132
display id: component1948132
version: 1
top level: BBa_I732731
definition: BBa_B0034
access: public

BBa_I732406
type: SequenceAnnotation
persistent identity: annotation1948124
display id: annotation1948124
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948124/range1948124/1
component: BBa_I732406

https://synbiohub.org/public/igem/BBa_I732731/annotation1948124/range1948124/1
type: Range
persistent identity: range1948124
display id: range1948124
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 1
end: 125
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948108
display id: annotation1948108
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948108/range1948108/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732731/annotation1948108/range1948108/1
type: Range
persistent identity: range1948108
display id: range1948108
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948110
display id: annotation1948110
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948110/range1948110/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732731/annotation1948110/range1948110/1
type: Range
persistent identity: range1948110
display id: range1948110
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948126
display id: annotation1948126
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948126/range1948126/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732731/annotation1948126/range1948126/1
type: Range
persistent identity: range1948126
display id: range1948126
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 134
end: 145
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948130
display id: annotation1948130
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948130/range1948130/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732731/annotation1948130/range1948130/1
type: Range
persistent identity: range1948130
display id: range1948130
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 152
end: 385
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948114
display id: annotation1948114
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948114/range1948114/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732731/annotation1948114/range1948114/1
type: Range
persistent identity: range1948114
display id: range1948114
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 288
end: 299
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1948117
display id: annotation1948117
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948117/range1948117/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I732731/annotation1948117/range1948117/1
type: Range
persistent identity: range1948117
display id: range1948117
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 306
end: 1025
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948132
display id: annotation1948132
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948132/range1948132/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732731/annotation1948132/range1948132/1
type: Range
persistent identity: range1948132
display id: range1948132
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 394
end: 405
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1948134
display id: annotation1948134
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948134/range1948134/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I732731/annotation1948134/range1948134/1
type: Range
persistent identity: range1948134
display id: range1948134
version: 1
top level: BBa_I732731
direction: direction/reverse
start: 412
end: 1131
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948118
display id: annotation1948118
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948118/range1948118/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732731/annotation1948118/range1948118/1
type: Range
persistent identity: range1948118
display id: range1948118
version: 1
top level: BBa_I732731
direction: direction/forward
start: 1034
end: 1113
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948120
display id: annotation1948120
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948120/range1948120/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732731/annotation1948120/range1948120/1
type: Range
persistent identity: range1948120
display id: range1948120
version: 1
top level: BBa_I732731
direction: direction/forward
start: 1122
end: 1162
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948135
display id: annotation1948135
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948135/range1948135/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732731/annotation1948135/range1948135/1
type: Range
persistent identity: range1948135
display id: range1948135
version: 1
top level: BBa_I732731
direction: direction/forward
start: 1140
end: 1219
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948137
display id: annotation1948137
version: 1
top level: BBa_I732731
location: https://synbiohub.org/public/igem/BBa_I732731/annotation1948137/range1948137/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732731/annotation1948137/range1948137/1
type: Range
persistent identity: range1948137
display id: range1948137
version: 1
top level: BBa_I732731
direction: direction/forward
start: 1228
end: 1268
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ alpha fragment
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 234
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 229
end: 234
orientation: inline

U037O11+D0
type: ComponentDefinition
persistent identity: BBa I732406
display id: BBa_I732406
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Family Member (U037O11+D062NUL)
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
mutable provenance: Might be later edited.
owned by: james
owned by: myers
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3K5. For more information please refer to BBa_I732403.
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Might be later edited.
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_I732007
sequence annotation: BglII
sequence annotation: BamHI
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BBa_I732006
sequence annotation: START
sequence annotation: lacZ
sequence annotation: STOP
sequence annotation: GFP protein
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I732406_sequence/1

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944462
display id: annotation1944462
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732406/annotation1944462/range1944462/1
type: Range
persistent identity: range1944462
display id: range1944462
version: 1
top level: U037O11+D0
direction: direction/reverse
start: 1
end: 19
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944460
display id: annotation1944460
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I732406/annotation1944460/range1944460/1
type: Range
persistent identity: range1944460
display id: range1944460
version: 1
top level: U037O11+D0
direction: direction/forward
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944461
display id: annotation1944461
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I732406/annotation1944461/range1944461/1
type: Range
persistent identity: range1944461
display id: range1944461
version: 1
top level: U037O11+D0
direction: direction/forward
start: 12
end: 17
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944464
display id: annotation1944464
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732406/annotation1944464/range1944464/1
type: Range
persistent identity: range1944464
display id: range1944464
version: 1
top level: U037O11+D0
direction: direction/reverse
start: 28
end: 39
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944463
display id: annotation1944463
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_I732406/annotation1944463/range1944463/1
type: Range
persistent identity: range1944463
display id: range1944463
version: 1
top level: U037O11+D0
direction: direction/forward
start: 32
end: 35
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944468
display id: annotation1944468
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732406/annotation1944468/range1944468/1
type: Range
persistent identity: range1944468
display id: range1944468
version: 1
top level: U037O11+D0
direction: direction/reverse
start: 46
end: 279
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944465
display id: annotation1944465
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I732406/annotation1944465/range1944465/1
type: Range
persistent identity: range1944465
display id: range1944465
version: 1
top level: U037O11+D0
direction: direction/forward
start: 46
end: 48
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944466
display id: annotation1944466
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732406/annotation1944466/range1944466/1
type: Range
persistent identity: range1944466
display id: range1944466
version: 1
top level: U037O11+D0
direction: direction/forward
start: 46
end: 279
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944467
display id: annotation1944467
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732406/annotation1944467/range1944467/1
type: Range
persistent identity: range1944467
display id: range1944467
version: 1
top level: U037O11+D0
direction: direction/forward
start: 274
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944470
display id: annotation1944470
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732406/annotation1944470/range1944470/1
type: Range
persistent identity: range1944470
display id: range1944470
version: 1
top level: U037O11+D0
direction: direction/reverse
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944469
display id: annotation1944469
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_I732406/annotation1944469/range1944469/1
type: Range
persistent identity: range1944469
display id: range1944469
version: 1
top level: U037O11+D0
direction: direction/forward
start: 292
end: 295
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944471
display id: annotation1944471
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_I732406/annotation1944471/range1944471/1
type: Range
persistent identity: range1944471
display id: range1944471
version: 1
top level: U037O11+D0
direction: direction/forward
start: 306
end: 1025
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944472
display id: annotation1944472
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732406/annotation1944472/range1944472/1
type: Range
persistent identity: range1944472
display id: range1944472
version: 1
top level: U037O11+D0
direction: direction/reverse
start: 306
end: 1025
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944473
display id: annotation1944473
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732406/annotation1944473/range1944473/1
type: Range
persistent identity: range1944473
display id: range1944473
version: 1
top level: U037O11+D0
direction: direction/forward
start: 1034
end: 1113
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944474
display id: annotation1944474
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I732406/annotation1944474/range1944474/1
type: Range
persistent identity: range1944474
display id: range1944474
version: 1
top level: U037O11+D0
direction: direction/forward
start: 1045
end: 1088
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944475
display id: annotation1944475
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732406/annotation1944475/range1944475/1
type: Range
persistent identity: range1944475
display id: range1944475
version: 1
top level: U037O11+D0
direction: direction/forward
start: 1122
end: 1162
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944476
display id: annotation1944476
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I732406/annotation1944476/range1944476/1
type: Range
persistent identity: range1944476
display id: range1944476
version: 1
top level: U037O11+D0
direction: direction/forward
start: 1129
end: 1148
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944477
display id: annotation1944477
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_I732406/annotation1944477/range1944477/1
type: Range
persistent identity: range1944477
display id: range1944477
version: 1
top level: U037O11+D0
direction: direction/forward
start: 1149
end: 1162
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944478
display id: annotation1944478
version: 1
top level: U037O11+D0
location: https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732406/annotation1944478/range1944478/1
type: Range
persistent identity: range1944478
display id: range1944478
version: 1
top level: U037O11+D0
direction: direction/forward
start: 1155
end: 1155
orientation: inline

https://synbiohub.org/public/igem/BBa_I732731_sequence/1
type: Sequence
persistent identity: BBa I732731 sequence
display id: BBa_I732731_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcgctcacaatttttacactttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaattaaacaggattaacaggatccggtactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732006_sequence/1
type: Sequence
persistent identity: BBa I732006 sequence
display id: BBa_I732006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732406_sequence/1
type: Sequence
persistent identity: BBa I732406 sequence
display id: BBa_I732406_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcgctcacaatttttacactttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaattaaacaggattaacaggatccgg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z