BBa_I732732
type: ComponentDefinition
persistent identity: BBa I732732
display id: BBa_I732732
version: 1
was generated by: iGEM to SBOL conversion
description: P_U097NUL+D002O22 + Double Reporters
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:59Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: N/A
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_B0034
component: BBa_I732006
component: BBa_I732407
sequence annotation: BBa_I732407
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732732_sequence/1

BBa_B0012
type: Component
persistent identity: component1948172
display id: component1948172
version: 1
top level: BBa_I732732
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1948170
display id: component1948170
version: 1
top level: BBa_I732732
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1948169
display id: component1948169
version: 1
top level: BBa_I732732
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1948167
display id: component1948167
version: 1
top level: BBa_I732732
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1948165
display id: component1948165
version: 1
top level: BBa_I732732
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1948161
display id: component1948161
version: 1
top level: BBa_I732732
definition: BBa_B0034
access: public

BBa_I732407
type: Component
persistent identity: component1948159
display id: component1948159
version: 1
top level: BBa_I732732
definition: U097NUL+D0
access: public

BBa_B0012
type: Component
persistent identity: component1948155
display id: component1948155
version: 1
top level: BBa_I732732
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1948153
display id: component1948153
version: 1
top level: BBa_I732732
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1948150
display id: component1948150
version: 1
top level: BBa_I732732
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1948146
display id: component1948146
version: 1
top level: BBa_I732732
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1948144
display id: component1948144
version: 1
top level: BBa_I732732
definition: BBa_B0034
access: public

BBa_I732407
type: SequenceAnnotation
persistent identity: annotation1948159
display id: annotation1948159
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948159/range1948159/1
component: BBa_I732407

https://synbiohub.org/public/igem/BBa_I732732/annotation1948159/range1948159/1
type: Range
persistent identity: range1948159
display id: range1948159
version: 1
top level: BBa_I732732
direction: direction/reverse
start: 1
end: 125
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948144
display id: annotation1948144
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948144/range1948144/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732732/annotation1948144/range1948144/1
type: Range
persistent identity: range1948144
display id: range1948144
version: 1
top level: BBa_I732732
direction: direction/reverse
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948146
display id: annotation1948146
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948146/range1948146/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732732/annotation1948146/range1948146/1
type: Range
persistent identity: range1948146
display id: range1948146
version: 1
top level: BBa_I732732
direction: direction/reverse
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948161
display id: annotation1948161
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948161/range1948161/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732732/annotation1948161/range1948161/1
type: Range
persistent identity: range1948161
display id: range1948161
version: 1
top level: BBa_I732732
direction: direction/reverse
start: 134
end: 145
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948165
display id: annotation1948165
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948165/range1948165/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732732/annotation1948165/range1948165/1
type: Range
persistent identity: range1948165
display id: range1948165
version: 1
top level: BBa_I732732
direction: direction/reverse
start: 152
end: 385
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948150
display id: annotation1948150
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948150/range1948150/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732732/annotation1948150/range1948150/1
type: Range
persistent identity: range1948150
display id: range1948150
version: 1
top level: BBa_I732732
direction: direction/reverse
start: 288
end: 299
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948167
display id: annotation1948167
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948167/range1948167/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732732/annotation1948167/range1948167/1
type: Range
persistent identity: range1948167
display id: range1948167
version: 1
top level: BBa_I732732
direction: direction/reverse
start: 394
end: 405
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1948169
display id: annotation1948169
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948169/range1948169/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I732732/annotation1948169/range1948169/1
type: Range
persistent identity: range1948169
display id: range1948169
version: 1
top level: BBa_I732732
direction: direction/reverse
start: 412
end: 1131
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948153
display id: annotation1948153
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948153/range1948153/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732732/annotation1948153/range1948153/1
type: Range
persistent identity: range1948153
display id: range1948153
version: 1
top level: BBa_I732732
direction: direction/forward
start: 1073
end: 1152
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948170
display id: annotation1948170
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948170/range1948170/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732732/annotation1948170/range1948170/1
type: Range
persistent identity: range1948170
display id: range1948170
version: 1
top level: BBa_I732732
direction: direction/forward
start: 1140
end: 1219
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948155
display id: annotation1948155
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948155/range1948155/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732732/annotation1948155/range1948155/1
type: Range
persistent identity: range1948155
display id: range1948155
version: 1
top level: BBa_I732732
direction: direction/forward
start: 1161
end: 1201
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948172
display id: annotation1948172
version: 1
top level: BBa_I732732
location: https://synbiohub.org/public/igem/BBa_I732732/annotation1948172/range1948172/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732732/annotation1948172/range1948172/1
type: Range
persistent identity: range1948172
display id: range1948172
version: 1
top level: BBa_I732732
direction: direction/forward
start: 1228
end: 1268
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ alpha fragment
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 234
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 229
end: 234
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732407
display id: BBa_I732407
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Family Member (U097NUL+D002O22)
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
mutable provenance: Might be later edited.
owned by: james
owned by: myers
mutable description: A Double Reporter (LacZ-alpha and GFP-AAV) loaded on pSB3C5. For more information please refer to BBa_I732401.
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Might be later edited.
star: false
creator: Zhan Jian
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I732007
sequence annotation: BglII
sequence annotation: BamHI
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: START
sequence annotation: lacZ
sequence annotation: BBa_I732006
sequence annotation: STOP
sequence annotation: BBa_E0044
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I732407_sequence/1

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944481
display id: annotation1944481
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732407/annotation1944481/range1944481/1
type: Range
persistent identity: range1944481
display id: range1944481
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 1
end: 19
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944479
display id: annotation1944479
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I732407/annotation1944479/range1944479/1
type: Range
persistent identity: range1944479
display id: range1944479
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944480
display id: annotation1944480
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I732407/annotation1944480/range1944480/1
type: Range
persistent identity: range1944480
display id: range1944480
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 12
end: 17
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944483
display id: annotation1944483
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732407/annotation1944483/range1944483/1
type: Range
persistent identity: range1944483
display id: range1944483
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 28
end: 39
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944482
display id: annotation1944482
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_I732407/annotation1944482/range1944482/1
type: Range
persistent identity: range1944482
display id: range1944482
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 32
end: 35
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944484
display id: annotation1944484
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732407/annotation1944484/range1944484/1
type: Range
persistent identity: range1944484
display id: range1944484
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 46
end: 48
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944485
display id: annotation1944485
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732407/annotation1944485/range1944485/1
type: Range
persistent identity: range1944485
display id: range1944485
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 46
end: 279
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944487
display id: annotation1944487
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732407/annotation1944487/range1944487/1
type: Range
persistent identity: range1944487
display id: range1944487
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 46
end: 279
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944486
display id: annotation1944486
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732407/annotation1944486/range1944486/1
type: Range
persistent identity: range1944486
display id: range1944486
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 274
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944489
display id: annotation1944489
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732407/annotation1944489/range1944489/1
type: Range
persistent identity: range1944489
display id: range1944489
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944488
display id: annotation1944488
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_I732407/annotation1944488/range1944488/1
type: Range
persistent identity: range1944488
display id: range1944488
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 292
end: 295
orientation: inline

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1944490
display id: annotation1944490
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732407/annotation1944490/range1944490/1
type: Range
persistent identity: range1944490
display id: range1944490
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 306
end: 1064
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944491
display id: annotation1944491
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732407/annotation1944491/range1944491/1
type: Range
persistent identity: range1944491
display id: range1944491
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1073
end: 1152
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944492
display id: annotation1944492
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I732407/annotation1944492/range1944492/1
type: Range
persistent identity: range1944492
display id: range1944492
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1084
end: 1127
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944493
display id: annotation1944493
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732407/annotation1944493/range1944493/1
type: Range
persistent identity: range1944493
display id: range1944493
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1161
end: 1201
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944494
display id: annotation1944494
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I732407/annotation1944494/range1944494/1
type: Range
persistent identity: range1944494
display id: range1944494
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1168
end: 1187
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944495
display id: annotation1944495
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_I732407/annotation1944495/range1944495/1
type: Range
persistent identity: range1944495
display id: range1944495
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1188
end: 1201
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944496
display id: annotation1944496
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732407/annotation1944496/range1944496/1
type: Range
persistent identity: range1944496
display id: range1944496
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1194
end: 1194
orientation: inline

https://synbiohub.org/public/igem/BBa_I732732_sequence/1
type: Sequence
persistent identity: BBa I732732 sequence
display id: BBa_I732732_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaaattgtaagcgcttacaattggatccggtactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732006_sequence/1
type: Sequence
persistent identity: BBa I732006 sequence
display id: BBa_I732006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732407_sequence/1
type: Sequence
persistent identity: BBa I732407 sequence
display id: BBa_I732407_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaaattgtaagcgcttacaattggatccgg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z