BBa_I732733
type: ComponentDefinition
persistent identity: BBa I732733
display id: BBa_I732733
version: 1
was generated by: iGEM to SBOL conversion
description: P_U097NUL+D014O22 + Double Reporters
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:59Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: N/A
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I732093
component: BBa_I732408
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_I732006
sequence annotation: BBa_I732408
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_I732093
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732733_sequence/1

BBa_I732093
type: Component
persistent identity: component2266129
display id: component2266129
version: 1
top level: BBa_I732733
definition: BBa_I732093
access: public

BBa_I732408
type: Component
persistent identity: component2266112
display id: component2266112
version: 1
top level: BBa_I732733
definition: U097NUL+D0
access: public

BBa_B0012
type: Component
persistent identity: component2266108
display id: component2266108
version: 1
top level: BBa_I732733
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2266106
display id: component2266106
version: 1
top level: BBa_I732733
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component2266101
display id: component2266101
version: 1
top level: BBa_I732733
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component2266097
display id: component2266097
version: 1
top level: BBa_I732733
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component2266095
display id: component2266095
version: 1
top level: BBa_I732733
definition: BBa_B0034
access: public

BBa_I732408
type: SequenceAnnotation
persistent identity: annotation2266112
display id: annotation2266112
version: 1
top level: BBa_I732733
location: https://synbiohub.org/public/igem/BBa_I732733/annotation2266112/range2266112/1
component: BBa_I732408

https://synbiohub.org/public/igem/BBa_I732733/annotation2266112/range2266112/1
type: Range
persistent identity: range2266112
display id: range2266112
version: 1
top level: BBa_I732733
direction: direction/reverse
start: 1
end: 137
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2266095
display id: annotation2266095
version: 1
top level: BBa_I732733
location: https://synbiohub.org/public/igem/BBa_I732733/annotation2266095/range2266095/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732733/annotation2266095/range2266095/1
type: Range
persistent identity: range2266095
display id: range2266095
version: 1
top level: BBa_I732733
direction: direction/reverse
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation2266097
display id: annotation2266097
version: 1
top level: BBa_I732733
location: https://synbiohub.org/public/igem/BBa_I732733/annotation2266097/range2266097/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732733/annotation2266097/range2266097/1
type: Range
persistent identity: range2266097
display id: range2266097
version: 1
top level: BBa_I732733
direction: direction/reverse
start: 46
end: 279
orientation: inline

BBa_I732093
type: SequenceAnnotation
persistent identity: annotation2266129
display id: annotation2266129
version: 1
top level: BBa_I732733
location: https://synbiohub.org/public/igem/BBa_I732733/annotation2266129/range2266129/1
component: BBa_I732093

https://synbiohub.org/public/igem/BBa_I732733/annotation2266129/range2266129/1
type: Range
persistent identity: range2266129
display id: range2266129
version: 1
top level: BBa_I732733
direction: direction/reverse
start: 146
end: 1280
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2266101
display id: annotation2266101
version: 1
top level: BBa_I732733
location: https://synbiohub.org/public/igem/BBa_I732733/annotation2266101/range2266101/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732733/annotation2266101/range2266101/1
type: Range
persistent identity: range2266101
display id: range2266101
version: 1
top level: BBa_I732733
direction: direction/reverse
start: 288
end: 299
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2266106
display id: annotation2266106
version: 1
top level: BBa_I732733
location: https://synbiohub.org/public/igem/BBa_I732733/annotation2266106/range2266106/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732733/annotation2266106/range2266106/1
type: Range
persistent identity: range2266106
display id: range2266106
version: 1
top level: BBa_I732733
direction: direction/forward
start: 1020
end: 1099
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2266108
display id: annotation2266108
version: 1
top level: BBa_I732733
location: https://synbiohub.org/public/igem/BBa_I732733/annotation2266108/range2266108/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732733/annotation2266108/range2266108/1
type: Range
persistent identity: range2266108
display id: range2266108
version: 1
top level: BBa_I732733
direction: direction/forward
start: 1108
end: 1148
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ alpha fragment
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 234
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 229
end: 234
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732408
display id: BBa_I732408
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Family Member (U097NUL+D014O22)
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
mutable provenance: Might be later edited.
owned by: james
owned by: myers
mutable description: A Double Reporter (LacZ-alpha and RFP) loaded on pSB3C5. For more information please refer to BBa_I732402.
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Might be later edited.
star: false
creator: Zhan Jian
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I732007
sequence annotation: BglII
sequence annotation: BamHI
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: lacZ
sequence annotation: START
sequence annotation: BBa_I732006
sequence annotation: STOP
sequence annotation: BBa_E1010
sequence annotation: mrfp1
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I732408_sequence/1

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944499
display id: annotation1944499
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732408/annotation1944499/range1944499/1
type: Range
persistent identity: range1944499
display id: range1944499
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 1
end: 19
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944497
display id: annotation1944497
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I732408/annotation1944497/range1944497/1
type: Range
persistent identity: range1944497
display id: range1944497
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944498
display id: annotation1944498
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I732408/annotation1944498/range1944498/1
type: Range
persistent identity: range1944498
display id: range1944498
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 12
end: 17
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944501
display id: annotation1944501
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732408/annotation1944501/range1944501/1
type: Range
persistent identity: range1944501
display id: range1944501
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 28
end: 39
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944500
display id: annotation1944500
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_I732408/annotation1944500/range1944500/1
type: Range
persistent identity: range1944500
display id: range1944500
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 32
end: 35
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944503
display id: annotation1944503
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732408/annotation1944503/range1944503/1
type: Range
persistent identity: range1944503
display id: range1944503
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 46
end: 279
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944502
display id: annotation1944502
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732408/annotation1944502/range1944502/1
type: Range
persistent identity: range1944502
display id: range1944502
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 46
end: 48
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944505
display id: annotation1944505
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732408/annotation1944505/range1944505/1
type: Range
persistent identity: range1944505
display id: range1944505
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 46
end: 279
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944504
display id: annotation1944504
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732408/annotation1944504/range1944504/1
type: Range
persistent identity: range1944504
display id: range1944504
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 274
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944507
display id: annotation1944507
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732408/annotation1944507/range1944507/1
type: Range
persistent identity: range1944507
display id: range1944507
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944506
display id: annotation1944506
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_I732408/annotation1944506/range1944506/1
type: Range
persistent identity: range1944506
display id: range1944506
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 292
end: 295
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1944509
display id: annotation1944509
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732408/annotation1944509/range1944509/1
type: Range
persistent identity: range1944509
display id: range1944509
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 306
end: 986
orientation: inline

mrfp1
type: SequenceAnnotation
persistent identity: annotation1944508
display id: annotation1944508
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732408/annotation1944508/range1944508/1
type: Range
persistent identity: range1944508
display id: range1944508
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 306
end: 980
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1944510
display id: annotation1944510
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732408/annotation1944510/range1944510/1
type: Range
persistent identity: range1944510
display id: range1944510
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 987
end: 1011
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944511
display id: annotation1944511
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732408/annotation1944511/range1944511/1
type: Range
persistent identity: range1944511
display id: range1944511
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1020
end: 1099
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944512
display id: annotation1944512
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I732408/annotation1944512/range1944512/1
type: Range
persistent identity: range1944512
display id: range1944512
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1031
end: 1074
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944513
display id: annotation1944513
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732408/annotation1944513/range1944513/1
type: Range
persistent identity: range1944513
display id: range1944513
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1108
end: 1148
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944514
display id: annotation1944514
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I732408/annotation1944514/range1944514/1
type: Range
persistent identity: range1944514
display id: range1944514
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1115
end: 1134
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944515
display id: annotation1944515
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_I732408/annotation1944515/range1944515/1
type: Range
persistent identity: range1944515
display id: range1944515
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1135
end: 1148
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944516
display id: annotation1944516
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732408/annotation1944516/range1944516/1
type: Range
persistent identity: range1944516
display id: range1944516
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1141
end: 1141
orientation: inline

BBa_I732093
type: ComponentDefinition
persistent identity: BBa I732093
display id: BBa_I732093
version: 1
was generated by: iGEM to SBOL conversion
description: Double Repoters (LacZ-alpha and GFP)
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: The activity of upstream promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_I732006
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732093_sequence/1

BBa_B0034
type: Component
persistent identity: component1947026
display id: component1947026
version: 1
top level: BBa_I732093
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1947024
display id: component1947024
version: 1
top level: BBa_I732093
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1947020
display id: component1947020
version: 1
top level: BBa_I732093
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1947031
display id: component1947031
version: 1
top level: BBa_I732093
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1947029
display id: component1947029
version: 1
top level: BBa_I732093
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1947028
display id: component1947028
version: 1
top level: BBa_I732093
definition: GFP
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947020
display id: annotation1947020
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947020/range1947020/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732093/annotation1947020/range1947020/1
type: Range
persistent identity: range1947020
display id: range1947020
version: 1
top level: BBa_I732093
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1947024
display id: annotation1947024
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947024/range1947024/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732093/annotation1947024/range1947024/1
type: Range
persistent identity: range1947024
display id: range1947024
version: 1
top level: BBa_I732093
direction: direction/reverse
start: 19
end: 252
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1947026
display id: annotation1947026
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947026/range1947026/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732093/annotation1947026/range1947026/1
type: Range
persistent identity: range1947026
display id: range1947026
version: 1
top level: BBa_I732093
direction: direction/reverse
start: 261
end: 272
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1947028
display id: annotation1947028
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947028/range1947028/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I732093/annotation1947028/range1947028/1
type: Range
persistent identity: range1947028
display id: range1947028
version: 1
top level: BBa_I732093
direction: direction/reverse
start: 279
end: 998
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1947029
display id: annotation1947029
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947029/range1947029/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732093/annotation1947029/range1947029/1
type: Range
persistent identity: range1947029
display id: range1947029
version: 1
top level: BBa_I732093
direction: direction/forward
start: 1007
end: 1086
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1947031
display id: annotation1947031
version: 1
top level: BBa_I732093
location: https://synbiohub.org/public/igem/BBa_I732093/annotation1947031/range1947031/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732093/annotation1947031/range1947031/1
type: Range
persistent identity: range1947031
display id: range1947031
version: 1
top level: BBa_I732093
direction: direction/forward
start: 1095
end: 1135
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_I732733_sequence/1
type: Sequence
persistent identity: BBa I732733 sequence
display id: BBa_I732733_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctttaaattgtaagcgcttacaattggatccggtactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732006_sequence/1
type: Sequence
persistent identity: BBa I732006 sequence
display id: BBa_I732006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732408_sequence/1
type: Sequence
persistent identity: BBa I732408 sequence
display id: BBa_I732408_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctttaaattgtaagcgcttacaattggatccgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732093_sequence/1
type: Sequence
persistent identity: BBa I732093 sequence
display id: BBa_I732093_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z