BBa_I732734
type: ComponentDefinition
persistent identity: BBa I732734
display id: BBa_I732734
version: 1
was generated by: iGEM to SBOL conversion
description: P_U097NUL+D026O22 + Double Reporters
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:59Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: N/A
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Zhan Jian
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_B0034
component: BBa_I732006
component: BBa_B0012
component: BBa_B0010
component: BBa_I732409
sequence annotation: BBa_I732409
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732734_sequence/1

BBa_E0040
type: Component
persistent identity: component1948242
display id: component1948242
version: 1
top level: BBa_I732734
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component1948240
display id: component1948240
version: 1
top level: BBa_I732734
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1948238
display id: component1948238
version: 1
top level: BBa_I732734
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1948234
display id: component1948234
version: 1
top level: BBa_I732734
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1948245
display id: component1948245
version: 1
top level: BBa_I732734
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1948243
display id: component1948243
version: 1
top level: BBa_I732734
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1948222
display id: component1948222
version: 1
top level: BBa_I732734
definition: BBa_B0034
access: public

BBa_I732006
type: Component
persistent identity: component1948218
display id: component1948218
version: 1
top level: BBa_I732734
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component1948216
display id: component1948216
version: 1
top level: BBa_I732734
definition: BBa_B0034
access: public

BBa_I732409
type: Component
persistent identity: component1948232
display id: component1948232
version: 1
top level: BBa_I732734
definition: U097NUL+D0
access: public

BBa_B0012
type: Component
persistent identity: component1948228
display id: component1948228
version: 1
top level: BBa_I732734
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1948226
display id: component1948226
version: 1
top level: BBa_I732734
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1948225
display id: component1948225
version: 1
top level: BBa_I732734
definition: GFP
access: public

BBa_I732409
type: SequenceAnnotation
persistent identity: annotation1948232
display id: annotation1948232
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948232/range1948232/1
component: BBa_I732409

https://synbiohub.org/public/igem/BBa_I732734/annotation1948232/range1948232/1
type: Range
persistent identity: range1948232
display id: range1948232
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 1
end: 149
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948216
display id: annotation1948216
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948216/range1948216/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732734/annotation1948216/range1948216/1
type: Range
persistent identity: range1948216
display id: range1948216
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 28
end: 39
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948218
display id: annotation1948218
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948218/range1948218/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732734/annotation1948218/range1948218/1
type: Range
persistent identity: range1948218
display id: range1948218
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948234
display id: annotation1948234
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948234/range1948234/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732734/annotation1948234/range1948234/1
type: Range
persistent identity: range1948234
display id: range1948234
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 158
end: 169
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1948238
display id: annotation1948238
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948238/range1948238/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732734/annotation1948238/range1948238/1
type: Range
persistent identity: range1948238
display id: range1948238
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 176
end: 409
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948222
display id: annotation1948222
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948222/range1948222/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732734/annotation1948222/range1948222/1
type: Range
persistent identity: range1948222
display id: range1948222
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 288
end: 299
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1948225
display id: annotation1948225
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948225/range1948225/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I732734/annotation1948225/range1948225/1
type: Range
persistent identity: range1948225
display id: range1948225
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 306
end: 1025
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948240
display id: annotation1948240
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948240/range1948240/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732734/annotation1948240/range1948240/1
type: Range
persistent identity: range1948240
display id: range1948240
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 418
end: 429
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1948242
display id: annotation1948242
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948242/range1948242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I732734/annotation1948242/range1948242/1
type: Range
persistent identity: range1948242
display id: range1948242
version: 1
top level: BBa_I732734
direction: direction/reverse
start: 436
end: 1155
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948226
display id: annotation1948226
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948226/range1948226/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732734/annotation1948226/range1948226/1
type: Range
persistent identity: range1948226
display id: range1948226
version: 1
top level: BBa_I732734
direction: direction/forward
start: 1034
end: 1113
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948228
display id: annotation1948228
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948228/range1948228/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732734/annotation1948228/range1948228/1
type: Range
persistent identity: range1948228
display id: range1948228
version: 1
top level: BBa_I732734
direction: direction/forward
start: 1122
end: 1162
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948243
display id: annotation1948243
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948243/range1948243/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732734/annotation1948243/range1948243/1
type: Range
persistent identity: range1948243
display id: range1948243
version: 1
top level: BBa_I732734
direction: direction/forward
start: 1164
end: 1243
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948245
display id: annotation1948245
version: 1
top level: BBa_I732734
location: https://synbiohub.org/public/igem/BBa_I732734/annotation1948245/range1948245/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732734/annotation1948245/range1948245/1
type: Range
persistent identity: range1948245
display id: range1948245
version: 1
top level: BBa_I732734
direction: direction/forward
start: 1252
end: 1292
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ alpha fragment
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 234
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 229
end: 234
orientation: inline

U097NUL+D0
type: ComponentDefinition
persistent identity: BBa I732409
display id: BBa_I732409
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Family Member (U097NUL+D026O22)
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:58Z
mutable provenance: Might be later edited.
owned by: james
owned by: myers
mutable description: A Double Reporter (LacZ-alpha and GFP) loaded on pSB3C5. For more information please refer to BBa_I732403.
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Might be later edited.
star: false
creator: Zhan Jian
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_I732007
sequence annotation: BglII
sequence annotation: BamHI
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: START
sequence annotation: lacZ
sequence annotation: BBa_I732006
sequence annotation: STOP
sequence annotation: BBa_E0040
sequence annotation: GFP protein
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I732409_sequence/1

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944519
display id: annotation1944519
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I732409/annotation1944519/range1944519/1
type: Range
persistent identity: range1944519
display id: range1944519
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 1
end: 19
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1944517
display id: annotation1944517
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I732409/annotation1944517/range1944517/1
type: Range
persistent identity: range1944517
display id: range1944517
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1944518
display id: annotation1944518
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I732409/annotation1944518/range1944518/1
type: Range
persistent identity: range1944518
display id: range1944518
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 12
end: 17
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944521
display id: annotation1944521
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732409/annotation1944521/range1944521/1
type: Range
persistent identity: range1944521
display id: range1944521
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 28
end: 39
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944520
display id: annotation1944520
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_I732409/annotation1944520/range1944520/1
type: Range
persistent identity: range1944520
display id: range1944520
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 32
end: 35
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1944522
display id: annotation1944522
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I732409/annotation1944522/range1944522/1
type: Range
persistent identity: range1944522
display id: range1944522
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 46
end: 48
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1944523
display id: annotation1944523
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732409/annotation1944523/range1944523/1
type: Range
persistent identity: range1944523
display id: range1944523
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 46
end: 279
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944525
display id: annotation1944525
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732409/annotation1944525/range1944525/1
type: Range
persistent identity: range1944525
display id: range1944525
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 46
end: 279
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1944524
display id: annotation1944524
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732409/annotation1944524/range1944524/1
type: Range
persistent identity: range1944524
display id: range1944524
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 274
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944527
display id: annotation1944527
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732409/annotation1944527/range1944527/1
type: Range
persistent identity: range1944527
display id: range1944527
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 288
end: 299
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation1944526
display id: annotation1944526
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_I732409/annotation1944526/range1944526/1
type: Range
persistent identity: range1944526
display id: range1944526
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 292
end: 295
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1944529
display id: annotation1944529
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732409/annotation1944529/range1944529/1
type: Range
persistent identity: range1944529
display id: range1944529
version: 1
top level: U097NUL+D0
direction: direction/reverse
start: 306
end: 1025
orientation: inline

GFP protein
type: SequenceAnnotation
persistent identity: annotation1944528
display id: annotation1944528
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I732409/annotation1944528/range1944528/1
type: Range
persistent identity: range1944528
display id: range1944528
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 306
end: 1025
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944530
display id: annotation1944530
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732409/annotation1944530/range1944530/1
type: Range
persistent identity: range1944530
display id: range1944530
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1034
end: 1113
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1944531
display id: annotation1944531
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_I732409/annotation1944531/range1944531/1
type: Range
persistent identity: range1944531
display id: range1944531
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1045
end: 1088
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944532
display id: annotation1944532
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I732409/annotation1944532/range1944532/1
type: Range
persistent identity: range1944532
display id: range1944532
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1122
end: 1162
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1944533
display id: annotation1944533
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_I732409/annotation1944533/range1944533/1
type: Range
persistent identity: range1944533
display id: range1944533
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1129
end: 1148
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1944534
display id: annotation1944534
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_I732409/annotation1944534/range1944534/1
type: Range
persistent identity: range1944534
display id: range1944534
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1149
end: 1162
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1944535
display id: annotation1944535
version: 1
top level: U097NUL+D0
location: https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732409/annotation1944535/range1944535/1
type: Range
persistent identity: range1944535
display id: range1944535
version: 1
top level: U097NUL+D0
direction: direction/forward
start: 1155
end: 1155
orientation: inline

https://synbiohub.org/public/igem/BBa_I732734_sequence/1
type: Sequence
persistent identity: BBa I732734 sequence
display id: BBa_I732734_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtaattgtaagcgcttacaattggatccggtactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732006_sequence/1
type: Sequence
persistent identity: BBa I732006 sequence
display id: BBa_I732006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732409_sequence/1
type: Sequence
persistent identity: BBa I732409 sequence
display id: BBa_I732409_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtaattgtaagcgcttacaattggatccgg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z