LOCUS       BBa_I732737             1328 bp    DNA     linear       22-Nov-2024
DEFINITION  P_U097NUL+D062O22 + Double Reporters
ACCESSION   BBa_I732737
VERSION     BBa_I732737.1  
FEATURES             Location/Qualifiers
     promoter        61..137
                     /label=consensus elements
     misc_feature    158..177
                     /label=O2
     misc_feature    137..137
                     /label=+1
     promoter        1..185
                     /label=BBa_I732412
     misc_feature    198..201
                     /label=conserved
     RBS             194..205
                     /label=BBa_B0034
     CDS             212..445
                     /label=lacZ
     misc_feature    212..214
                     /label=START
     misc_feature    440..445
                     /label=STOP
     CDS             212..445
                     /label=BBa_I732006
     misc_feature    458..461
                     /label=conserved
     RBS             454..465
                     /label=BBa_B0034
     CDS             472..1191
                     /label=GFP protein
     CDS             472..1191
                     /label=BBa_E0040
     stem_loop       1211..1254
                     /label=stem_loop
     misc_feature    1200..1279
                     /label=BBa_B0010
     terminator      1200..1279
                     /label=BBa_B0010
     stem_loop       1295..1314
                     /label=T7 TE
     misc_feature    1321..1321
                     /label=stop
     polyA_site      1315..1328
                     /label=polya
     misc_feature    1288..1328
                     /label=BBa_B0012
     terminator      1288..1328
                     /label=BBa_B0012
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaaca tcccctttat ctatcgcctg
      121 tgcccactac atcaagccaa attaaacagg attaacaaat tgtaagcgct tacaattgga
      181 tccggtacta gagaaagagg agaaatacta gatgaccatg attacggatt cactggccgt
      241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      361 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
      421 gccggaaagc tggctggagt aataatacta gagaaagagg agaaatacta gatgcgtaaa
      481 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      541 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      601 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      661 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      721 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      781 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      841 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      901 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      961 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     1021 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     1081 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     1141 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
     1201 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1261 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1321 cgtttata
//