LOCUS       BBa_I732743             1253 bp    DNA     linear       21-May-2024
DEFINITION  P_D01O21 + Double Reporters (lacZ+RFP)
ACCESSION   BBa_I732743
VERSION     BBa_I732743.1  
FEATURES             Location/Qualifiers
     promoter        1..124
                     /label=BBa_I732273
     misc_feature    137..140
                     /label=conserved
     RBS             133..144
                     /label=BBa_B0034
     CDS             151..384
                     /label=lacZ
     misc_feature    151..153
                     /label=START
     misc_feature    379..384
                     /label=STOP
     CDS             151..384
                     /label=BBa_I732006
     misc_feature    133..384
                     /label=BBa_I732018
     misc_feature    397..400
                     /label=conserved
     RBS             393..404
                     /label=BBa_B0034
     CDS             411..1085
                     /label=mrfp1
     misc_feature    1092..1116
                     /label=Help:Barcodes
     CDS             411..1116
                     /label=BBa_E1010
     stem_loop       1136..1179
                     /label=stem_loop
     misc_feature    1125..1204
                     /label=BBa_B0010
     terminator      1125..1204
                     /label=BBa_B0010
     stem_loop       1220..1239
                     /label=T7 TE
     misc_feature    1246..1246
                     /label=stop
     polyA_site      1240..1253
                     /label=polya
     misc_feature    1213..1253
                     /label=BBa_B0012
     terminator      1213..1253
                     /label=BBa_B0012
     terminator      1125..1253
                     /label=BBa_B0015
     misc_feature    133..1253
                     /label=BBa_I732092
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtaagcgctc acaattggat
      121 ccggtactag agaaagagga gaaatactag atgaccatga ttacggattc actggccgtc
      181 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      241 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      301 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
      361 ccggaaagct ggctggagta ataatactag agaaagagga gaaatactag atggcttcct
      421 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
      481 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
      541 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
      601 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
      661 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
      721 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
      781 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
      841 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
      901 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
      961 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
     1021 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
     1081 gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa
     1141 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1201 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//