##gff-version 3
##sequence-region BBa_I732744 1 1253
BBa_I732744	.	promoter	1	124	.	+	0	ID=BBa_I732279;Name=BBa_I732279
BBa_I732744	.	engineered_region	133	1253	.	+	0	ID=BBa_I732092;Name=BBa_I732092
BBa_I732744	.	ribosome_entry_site	393	404	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732092
BBa_I732744	.	conserved_region	397	400	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732744	.	engineered_region	133	384	.	+	0	ID=BBa_I732018;Name=BBa_I732018;Parent=BBa_I732092
BBa_I732744	.	ribosome_entry_site	133	144	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732018
BBa_I732744	.	conserved_region	137	140	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732744	.	CDS	151	384	.	+	0	ID=BBa_I732006;Name=BBa_I732006;Parent=BBa_I732018
BBa_I732744	.	CDS	151	384	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_I732744	.	start_codon	151	153	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_I732744	.	stop_codon	379	384	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_I732744	.	terminator	1125	1253	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I732092
BBa_I732744	.	terminator	1125	1204	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I732744	.	engineered_region	1125	1204	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732744	.	stem_loop	1136	1179	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732744	.	terminator	1213	1253	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I732744	.	engineered_region	1213	1253	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732744	.	polyA_site	1240	1253	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732744	.	stop_codon	1246	1246	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732744	.	stem_loop	1220	1239	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732744	.	CDS	411	1116	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I732092
BBa_I732744	.	CDS	411	1085	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I732744	.	engineered_tag	1092	1116	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_I732744
tacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacactttatgcttccg
gctcgtataatgtgtggaattatgagcgctcacaattggatccggtactagagaaagaggagaaatactagatgaccat
gattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgca
gcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctga
atggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagaaa
gaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccg
ttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagt
taccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaa
cacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaag
acggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtac
caacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaa
gacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaacca
cctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacga
agactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctag
tgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata