LOCUS       BBa_I732745             1294 bp    DNA     linear       22-Nov-2024
DEFINITION  P_U097NUL+D062NUL + Double Reporters (lacZ+RFP)
ACCESSION   BBa_I732745
VERSION     BBa_I732745.1  
FEATURES             Location/Qualifiers
     promoter        1..165
                     /label=BBa_I732400
     misc_feature    178..181
                     /label=conserved
     RBS             174..185
                     /label=BBa_B0034
     CDS             192..425
                     /label=lacZ
     misc_feature    192..194
                     /label=START
     misc_feature    420..425
                     /label=STOP
     CDS             192..425
                     /label=BBa_I732006
     misc_feature    174..425
                     /label=BBa_I732018
     misc_feature    438..441
                     /label=conserved
     RBS             434..445
                     /label=BBa_B0034
     CDS             452..1126
                     /label=mrfp1
     misc_feature    1133..1157
                     /label=Help:Barcodes
     CDS             452..1157
                     /label=BBa_E1010
     stem_loop       1177..1220
                     /label=stem_loop
     misc_feature    1166..1245
                     /label=BBa_B0010
     terminator      1166..1245
                     /label=BBa_B0010
     stem_loop       1261..1280
                     /label=T7 TE
     misc_feature    1287..1287
                     /label=stop
     polyA_site      1281..1294
                     /label=polya
     misc_feature    1254..1294
                     /label=BBa_B0012
     terminator      1254..1294
                     /label=BBa_B0012
     terminator      1166..1294
                     /label=BBa_B0015
     misc_feature    174..1294
                     /label=BBa_I732092
ORIGIN
        1 tacccacaac ccaattcgag accggaactc gattgtatct gtagtgcttt agtagtggag
       61 tttacacttt atgcttccgg ctcgtataat gtgtggaaca tcccctttat ctatcgcctg
      121 tgcccactac atcaagccaa attaaacagg attaacagga tccggtacta gagaaagagg
      181 agaaatacta gatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact
      241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      361 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
      421 aataatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga
      481 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
      541 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
      601 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
      661 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
      721 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga
      781 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
      841 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
      901 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
      961 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
     1021 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
     1081 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata
     1141 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1201 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1261 ggctcacctt cgggtgggcc tttctgcgtt tata
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