LOCUS       BBa_I732752             1324 bp    DNA     linear       26-Dec-2024
DEFINITION  P_U073O26+D062O61 + Double Reporters
ACCESSION   BBa_I732752
VERSION     BBa_I732752.1  
FEATURES             Location/Qualifiers
     promoter        1..181
                     /label=BBa_I732452
     misc_feature    194..197
                     /label=conserved
     RBS             190..201
                     /label=BBa_B0034
     CDS             208..441
                     /label=lacZ
     misc_feature    208..210
                     /label=START
     misc_feature    436..441
                     /label=STOP
     CDS             208..441
                     /label=BBa_I732006
     misc_feature    454..457
                     /label=conserved
     RBS             450..461
                     /label=BBa_B0034
     CDS             468..1187
                     /label=GFP protein
     CDS             468..1187
                     /label=BBa_E0040
     stem_loop       1207..1250
                     /label=stem_loop
     misc_feature    1196..1275
                     /label=BBa_B0010
     terminator      1196..1275
                     /label=BBa_B0010
     stem_loop       1291..1310
                     /label=T7 TE
     misc_feature    1317..1317
                     /label=stop
     polyA_site      1311..1324
                     /label=polya
     misc_feature    1284..1324
                     /label=BBa_B0012
     terminator      1284..1324
                     /label=BBa_B0012
ORIGIN
        1 aattgtaagc gctcataatt gaactcgatt gtatctgtag tgctttagta gtggagttta
       61 cactttatgc ttccggctcg tataatgtgt ggaacatccc ctttatctat cgcctgtgcc
      121 cactacatca agccaaatta aacaggatta acaaattatg agcgctcaca attggatccg
      181 gtactagaga aagaggagaa atactagatg accatgatta cggattcact ggccgtcgtt
      241 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      301 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      361 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
      421 gaaagctggc tggagtaata atactagaga aagaggagaa atactagatg cgtaaaggag
      481 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      541 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      601 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      661 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      721 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      781 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      841 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      901 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      961 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1021 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1081 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1141 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
     1201 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1261 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1321 tata
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