LOCUS BBa_I732901 3438 bp DNA linear 22-Nov-2024 DEFINITION R0010 I732019 ACCESSION BBa_I732901 VERSION BBa_I732901.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 3250..3250 /label=C->T CDS 227..3301 /label=lacZ misc_feature 227..229 /label=START misc_feature 3299..3301 /label=STOP CDS 227..3301 /label=BBa_I732005 stem_loop 3321..3364 /label=stem_loop misc_feature 3310..3389 /label=BBa_B0010 terminator 3310..3389 /label=BBa_B0010 stem_loop 3405..3424 /label=T7 TE misc_feature 3431..3431 /label=stop polyA_site 3425..3438 /label=polya misc_feature 3398..3438 /label=BBa_B0012 terminator 3398..3438 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ccatgattac 241 ggattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 301 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 361 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc 421 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt 481 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac 541 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc 601 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga 661 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga 721 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct 781 cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg 841 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga 901 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt 961 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga 1021 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg 1081 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga 1141 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga 1201 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct 1261 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg 1321 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt 1381 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg 1441 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa 1501 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt 1561 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca 1621 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt 1681 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc 1741 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct 1801 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa 1861 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg 1921 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc 1981 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa 2041 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca 2101 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt 2161 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc 2221 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga 2281 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa 2341 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga 2401 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga 2461 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt 2521 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt 2581 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa 2641 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca 2701 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca 2761 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat 2821 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct 2881 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga 2941 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga 3001 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt 3061 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca 3121 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct 3181 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc 3241 ggcggaattt cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata 3301 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3361 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3421 ggcctttctg cgtttata //