LOCUS       BBa_I732913             1757 bp    DNA     linear       16-Feb-2025
DEFINITION  [aTC] -> RFP
ACCESSION   BBa_I732913
VERSION     BBa_I732913.1  
FEATURES             Location/Qualifiers
     misc_feature    1..689
                     /label=BBa_I732084
     stem_loop       709..752
                     /label=stem_loop
     misc_feature    698..777
                     /label=BBa_B0010
     terminator      698..777
                     /label=BBa_B0010
     stem_loop       793..812
                     /label=T7 TE
     misc_feature    819..819
                     /label=stop
     polyA_site      813..826
                     /label=polya
     misc_feature    786..826
                     /label=BBa_B0012
     terminator      786..826
                     /label=BBa_B0012
     misc_feature    835..853
                     /label=TetR 1
     misc_feature    835..888
                     /label=BBa_R0040
     promoter        854..859
                     /label=-35
     misc_feature    860..878
                     /label=TetR 2
     promoter        877..882
                     /label=-10
     promoter        835..888
                     /label=BBa_R0040
     misc_feature    1..888
                     /label=BBa_I732086
     misc_feature    901..904
                     /label=conserved
     RBS             897..908
                     /label=BBa_B0034
     CDS             915..1589
                     /label=mrfp1
     misc_feature    1596..1620
                     /label=Help:Barcodes
     CDS             915..1620
                     /label=BBa_E1010
     stem_loop       1640..1683
                     /label=stem_loop
     misc_feature    1629..1708
                     /label=BBa_B0010
     terminator      1629..1708
                     /label=BBa_B0010
     stem_loop       1724..1743
                     /label=T7 TE
     misc_feature    1750..1750
                     /label=stop
     polyA_site      1744..1757
                     /label=polya
     misc_feature    1717..1757
                     /label=BBa_B0012
     terminator      1717..1757
                     /label=BBa_B0012
     terminator      1629..1757
                     /label=BBa_B0015
ORIGIN
        1 tggtgcaaaa cctttgcggt atggcatgat agcgcctact agagaaagag gagaaatact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tcctaataat actagagcca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      841 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag
      901 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag
      961 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc
     1021 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt
     1081 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc
     1141 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg
     1201 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg
     1261 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta
     1321 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg
     1381 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg
     1441 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg
     1501 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac
     1561 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc
     1621 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1681 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1741 gcctttctgc gtttata
//